Resolute® Liquid Chromatography Columns
Precision and Automation Combined for Secure, Efficient Manufacturing
Commercial manufacturing of any therapeutic must balance two key criteria:
- Reliability – reproducible process quality to ensure a safe, consistent product
- Efficiency – minimal cost per dose without compromising efficacy or quality
Sartorius’ low-pressure liquid chromatography (LPLC) columns are a proven solution to meet both needs:
- Precise linear actuation delivers highly repeatable packing and optimal resolution throughout the chromatography process
- One-button automation reduces human error, downtime, and buffer consumption while maximizing packing success and resin utilization
Liquid Chromatography Columns Portfolio
Sartorius offers both manual and fully automated columns in diameters from 300 to 2,000 mm, supporting pilot to commercial-scale applications. Backed by extensive design and engineering expertise, our portfolio can accommodate a wide range of process requirements. A key innovation is the patented nozzle design, which enables robust, pack-in-place functionality.
| Attribute | Resolute® ManuPak | Resolute® AutoPak |
|---|---|---|
| Column Diameter | 300-1,000 mm (up to 2,000 mm optionally) | 400-2,000 mm |
| Application | Packed-bed chromatography processes ideal for large molecules such as mAbs, ADCs, vaccines, and blood or plasma-derived products | |
| Automated Operations | Manual column packing | Fully automated packing, unpacking, CIP and in-place repacking |
| Tube Material | Stainless-steel | acrylic | Stainless-steel | acrylic |
| Max Operating Pressure | 5 barg | 4 barg | 5 barg | 4 barg |
| Custom Engineer-To-Order (ETO) Capabilities | Yes | Yes |
| View Products | Consult Our Experts | |
Resolute® ManuPak columns provide a cost-effective solution with a proven flow path design and configurable options to support individual process needs and operational flexibility. The Resolute® column range has been trusted in the industry for over 20 years, with more than 1,500 installations.
- Pack-in-place capability in a closed system reduces operational risk
- High-resolution flow path offers an optimal balance between pressure drop and chromatography performance
- Direct scalability to the Resolute® AutoPak product line minimizes process validation effort
- A range of options available to meet unique process requirements
Resolute® AutoPak columns enable fully automated packing, repacking, unpacking, and CIP operations—eliminating many of the common pain points associated with packed bed chromatography and maximizing process uptime. Columns are available in internal diameters (ID) ranging from 400 mm to 2,000 mm.
- Fully automated “one-button” operation ensures repeatable, robust performance
- Enables complete resin utilization
- Air-assisted unpacking reduces buffer consumption
- Repack-in-place functionality preserves valuable media and enables fast turnaround
Supporting Products & Services
Learn More About Liquid Chromatography Columns
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A Trusted Partner for Chromatography Columns
With over 20 years of engineer-to-order column expertise for some of the world’s largest pharmaceutical manufacturers, Sartorius combines deep design and engineering capabilities with proven reliability to meet diverse process requirements. This core team is supported by a global network of chromatography specialists with extensive experience in chromatography processes and column packing.
- More than 1,500 Resolute® columns installed worldwide, including for commercial production of multiple blockbuster therapeutics
- Wet demonstrations through our field applications network
- Column packing demonstrations and customer-specific packing trials
- Support for recipe development and qualification documentation
- Hands-on customer training
- Expert support throughout the entire lifecycle of the hardware
Frequently Asked Questions
The main advantage of Resolute® AutoPak over alternative manually packed columns is the reliability of the column packing. A failed pack can lead to wasted resin and labor costs, plus a delay in production. Automated packing reduces the risk, providing significant cost savings and risk mitigation over the column's lifetime.
The advantages of Resolute® AutoPak over alternative manually packed columns are the reliability of the column packing. A failed pack can lead to wasted resin and labor costs, plus a delay in production. Automated packing reduces the risk, therefore providing a significant cost savings and risk mitigation over the column's lifetime.
Resolute® AutoPak columns have automatic piston control, which eliminates column damage from skew. This ensures consistent column packing, removing batch-to-batch variability in the chromatography step.
Sartorius experts were the original designers of the automated chromatography column, bringing over 20 years of experience in column design and manufacturing. More than 1,500 Resolute® columns have been installed globally and are trusted for the commercial production of multiple blockbuster therapeutics.
