Dynamic Axial Compression Columns
High Performance Liquid Chromatography (HPLC) Columns
Hipersep® Prochrom columns are designed to meet the highest standards of performance, ensuring both efficiency and scalability. Dynamic axial compression (DAC) technology, combined with advanced packing methodology, delivers reproducibility and long-term robustness. Our packing technology has been developed to achieve a well-packed bed in minimal time, providing optimal results for smaller molecular weight therapeutics, which commonly require chromatography media with particle sizes of 10 to 15 μm. The columns are designed for easy cleaning and maintenance while maximizing operator safety. They enable:
- Efficiency and scalability from lab to industrial scale
- Fast and safe packing and unpacking — complete packing in 15–30 minutes
- Maximum operating pressure: 100 bar
Dynamic Axial Compression Columns Portfolio
From Development to Commercial Manufacturing
Our columns are designed for ease of cleaning and maintenance operations while maximizing operator safety. DAC technology enables performance consistency over time, meaning Hipersep® Prochrom columns can run for decades without any performance loss. Some columns installed in production plants in the 1980s are still operating today.
| Scale | Process Development | Clinical | Clinical | Commercial Manufacturing | Commercial Manufacturing |
|---|---|---|---|
| Inner diameter | 50 | 80 | 110 | 150 mm | 110 | 150 | 200 | 300 | 450 | 590 mm | 590 | 800 | 1,000 | 1,200 mm |
| Average production capacity | Gram to few kilograms per year | Kilograms per year | Kilograms to tons per year |
| Standards and norms | Explosion-proof ATEX and Class 1 Div 2, CODAP | ASME | ||
| Main application | Chromatography for APIs, peptides, oligonucleotides, insulin, GLP-1, and other small-molecular-weight therapeutics | ||
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Robust HPLC With Hipersep® Prochrom DAC
Preparative HPLC is a technique used to separate and purify compounds from mixtures on a larger scale. Unlike analytical HPLC, which is used to identify and quantify components in a sample, preparative HPLC focuses on isolating and collecting significant quantities of a specific compound. This method is the preferred choice for purifying peptides, insulin, and oligonucleotides with very high purity and yield.
Scaling up preparative HPLC presents several challenges that require careful consideration and optimization. One key issue is the need for larger columns, which can make it difficult to maintain uniform packing and flow distribution, potentially reducing separation efficiency. Hipersep® Prochrom DAC technology provides consistent and reproducible packing performance over time, allowing you to achieve the same resolution and purity as lab-scale operations while ensuring regulatory and GMP compliance.
Frequently Asked Questions
Preparative high-performance liquid chromatography (HPLC) is a specialized technique used for the large-scale purification and isolation of active pharmaceutical ingredients (APIs) and other valuable compounds. Unlike analytical HPLC, which focuses on identifying and quantifying components, preparative HPLC is designed to collect significant quantities of a specific compound with high purity and yield. This method is essential for producing bulk quantities of drugs and intermediates, ensuring that they meet stringent regulatory standards for purity and quality. In the pharmaceutical industry, preparative HPLC is a preferred choice for purifying complex mixtures, such as peptides, insulin, and oligonucleotides, due to its ability to maintain consistency and reproducibility while scaling up production processes.
Dynamic axial compression (DAC) columns are specialized chromatography columns used in preparative high-performance liquid chromatography (HPLC) to enhance separation efficiency and reproducibility. These columns employ a DAC mechanism, maintaining uniform packing density and minimizing voids within the column bed during operation. This technology ensures consistent flow distribution and optimal interaction between the stationary and the mobile phase, improving the resolution and purity of separated compounds. DAC columns are particularly beneficial in large-scale purification processes, such as those in the pharmaceutical industry, where maintaining high performance and reliability is crucial.
The primary difference between preparative and analytical chromatography lies in their objectives and scale. Preparative chromatography is focused on isolating and purifying large quantities of specific compounds for production purposes, emphasizing high yield and purity. In contrast, analytical chromatography is used to identify, quantify, and analyze components within a sample, prioritizing precision and accuracy over quantity. Preparative chromatography operates on a larger scale, while analytical chromatography is typically conducted on a smaller scale for detailed analysis.
Preparative high-performance liquid chromatography (HPLC) is primarily used for the purification and isolation of significant quantities of specific compounds from complex mixtures. It is essential in industries such as pharmaceuticals, biotechnology, and chemical manufacturing, where high purity and yield are required for active pharmaceutical ingredients (APIs), intermediates, and other valuable substances. Preparative HPLC is the method of choice for purifying compounds like peptides, insulin, GLP-1, oligonucleotides, and natural extracts, ensuring they meet stringent regulatory standards for quality.
