eBook: Organoid Analysis

 Last updated: November 2023

Overview

The application of organoids as in vitro models is increasingly gaining popularity. These models have the ability to accurately replicate in vivo pathophysiology, thereby making them an essential instrument for the investigation of human development and disease.

Nevertheless, in order to maximize their potential, it is crucial to minimize variability and establish technological pipelines that can precisely image, monitor, and quantify these intricate 3D cell models.

Numerous existing methods for characterizing and visualizing organoids have low throughput and are not scalable for screening, necessitating labor-intensive, costly, and manual procedures to capture organoid images. Additionally, these current techniques frequently depend on third-party software for endpoint analysis or the incorporation of fluorescent markers. These markers only offer limited quantitative data and may also disrupt biological responses.


  • Document type: eBook
  • Page count: 39
  • Read time: 60 minutes


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Explore various methods for characterizing and analyzing organoids including:

  • A culture platform for tumor organoids with a novel geometrical format to facilitate high-throughput screening
  • A fluorometric method to detect cell death
  • Label-free, real-time live-cell analysis of 3D organoids in Matrigel®

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