Optimization of iPSC Culture Protocol Using High Quality Growth Factors and Cytokines
Authors: Daryl Cole, PhD — Scientist – Sartorius, Amber Ward — Scientist – Sartorius, Nicola Bevan, PhD - Sartorius | Last updated: November 2023
Overview
Growth factors and cytokines constitute a diverse group of proteins essential for intercellular communication. Two cytokines that are a key focus for in vitro cell culture are fibroblast growth factor 2 (FGF-2) and transforming growth factor β1 (TGF-β1). These cytokines are used in precise concentrations for maintaining induced pluripotent stem cells (iPSCs) in their pluripotent state. However, achieving the desired efficacy of these cytokines and growth factors in an in vitro setting has been challenging due to issues like thermostability, leading to a short half-life and necessitating frequent media changes, which are resource intensive.
In this application note, we present data on the utilization and optimization of Sartorius Research Use Only (RUO) Cytokines and Growth Factors for iPSC culture. Our goal is to sustain pluripotency and proliferative potential while reducing the need for daily media replenishment. We assess this by analyzing crucial cell surface markers, morphological indicators, and growth patterns using advanced tools like the iQue® Advanced Flow Cytometer and the Incucyte® Live-Cell Analysis System, showcasing the practicality of Sartorius RUO Growth Factors and Cytokines in iPSC culture maintenance.
- Document type: Application note
- Page count: 10
- Read time: 20 minutes
Key Takeaways
- How to effectively use Sartorius RUO Growth Factors and Cytokines as supplements in iPSC media formulations to preserve pluripotency, support growth, and increase the interval between feedings
- How to use the iQue® Advanced Flow Cytometer Platform in conjunction with the Incucyte® Live-Cell Analysis System for simple monitoring and quantification of growth and pluripotent phenotype of iPSC cells during cell culture and media formulation development
Optimization of iPSC Culture Protocol Using High Quality Growth Factors and Cytokines
