Optimization of SH-SY5Y Differentiation Using Growth Factors and Cytokines
Authors: Jasmine Trigg, Daryl Cole, Natasha Lewis, Nicola Bevan, Tim Dale | Last updated: July 2023
Overview
For neuroscientific research a crucial component is utilizing appropriate model systems which are translational for studying human development and disease. The use of in vitro models has greatly enhanced the field and traditionally these include primary rodent neuronal cultures, end-point slice cultures, and immortalized cell lines.
Growth factors and cytokines are signaling molecules that play critical roles in these models and are involved in a wide range of functions including cell growth, differentiation, and survival.
In this application note, we demonstrate robust differentiation of human SH-SY5Y cells into neuronal-like cells using Sartorius Research Use Only (RUO) Growth Factors and Cytokines and the utility of Incucyte® Live-Cell Imaging and Analysis to kinetically visualize and quantify changes in proliferation, morphology, and cell surface antigen expression.
- Document type: Application Note
- Page count: 12
- Read time: 15 minutes
Key Takeaways
- Discover robust, translational in vitro models that accurately recapitulate desired phenotypes
- Explore a powerful approach for reproducibly generating in vitro neuronal cultures
- Learn more about an efficient platform to investigate biological mechanisms underlying specific phenomena
Quick guide of Incucyte® Live-Cell Immunocytochemistry (ICC) protocol
