SoloHill® Microcarriers
Animal Protein Coated and Animal Product Free Microcarriers
SoloHill® Microcarriers promote excellent anchorage-dependent cell line attachment and growth and have been used for the production of animal and human health products for over 30 years. Without hydration or pre-swelling needed, microcarriers are easily sterilized for use, and their solid core design simplifies seed train preparation. Available both in animal protein-coated and animal product free, SoloHill® Microcarriers fit into any process and are an excellent choice for scale-up projects where process development timelines and capital investment are limiting.
Microcarriers - Find the Right Solution
SoloHill® Animal Product Free Microcarriers are for researchers working in limited or without animal sources in their processes. Sartorius offers a high performing certified animal-free microcarrier, perfect for researchers who desire to work within a completely serum-free media and animal product free (APF) process.
Description | Modified Polystyrene | Cross-linked Polystyrene | Cross-linked Polystyrene | Cross-linked Polystyrene |
Size Range | 160 – 200 µm | 90 - 150 & 125 - 212 µm | 125 - 212 µm | 125 - 212 µm |
Specific Gravity | 1.090 - 1.150 | 1.022 - 1.030 & 1.034 - 1.046 | 1.022 - 1.030 | 1.020 - 1.035 |
Area/Gram | 515 cm2 | 480 & 360 cm2 | 360 cm2 | 360 cm2 |
Surface Charge | Yes | No | Yes | Yes |
Animal collagen has proven to be a useful promoter of cell attachment and growth in monolayer cultures of adherent cell lines. SoloHill® animal protein coated microcarriers can be easily moved between collagen-based monolayers to more space-efficient 3D cultures and are ideal for cell attachment kinetics across a wide variety of tested cell lines.
Description | Cross-linked Polystyrene | Cross-linked Polystyrene |
Animal Protein | Yes - Type 1 porcine collagen coating | Yes – Surface modified Type 1 porcine collagen coating |
Size Range | 90 - 150 & 125 - 212 µm | 125 - 212 µm |
Specific Gravity | 1.022 - 1.030 & 1.034 - 1.046 | 1.022 - 1.030 |
Area/Gram | 480 & 360 cm2 | 360 cm2 |
Surface Charge | No | Yes |
SoloHill® Microcarrier Applications
Cell culture and tissue culture scientists worldwide have reported success using SoloHill microcarriers. Literature references and researcher feedback conclusively demonstrate flexibility in multiple adherent applications.
Researcher Reported Applications
Cell Line | Description | Charged Collagen Coated | |||
|---|---|---|---|---|---|
VERO | monkey kidney | x | x | x | |
CEF | chick embryo fibroblast | x | |||
ST | swine testes | x | |||
BT | bovine turbinate | x | |||
P4RSF | rabbit skin fibroblast | x | |||
MRC-5 | human diploid fibroblast | x | |||
KB | human epithelial carcinoma | x | |||
HFF | human foreskin fibroblast | x | x | x | |
UMSCC | human squamous carcinoma | x | x | x | x |
MDBK | Madin-Darby bovine kidney | x | x | ||
CHO | chinese hamster ovary | x | |||
MDCK | Madin-Darby canine kidney | x | |||
hMSC | Human Mesenchymal Stem Cells | x | x |
SoloHill® Microcarriers for Therapeutics and Vaccines
SoloHill® microcarriers have been used in the commercial production of both human and animal biopharmaceutical products.
FDA-licensed human therapeutics:
- Thyroid cancer diagnostic
- Thyroid cancer therapy
- Beta interferon – MS indication
USDA-licensed animal vaccines:
- Equine influenza
- Canine parvovirus
- Feline leukemia and many others
SoloHill scientists are actively researching new applications. This research has led to completed development on novel human vaccine protocols.
- Human influenza vaccine
- Marek's disease vaccine
The cationic amine-modified microporous surface of the Hillex® microcarriers with polystyrene core improves cell attraction and attachment without affecting cell harvest efficiency.
Compared to porous microcarriers, styrene copolymer microcarriers are constructed from solid non-toxic plastic which prevents absorption of toxic byproducts and makes harvesting simple with cell viabilities typically greater than 95%. The plastic beads allow for easy process transfer out of traditional styrene labware methods such as roller bottles and monolayers.
Incorporated with an electric charge, the Plastic Plus microcarriers strengthen cell attraction and attachment. Like the non-toxic Plastic microcarriers, Plastic Plus are solidly constructed from styrene copolymer, and harvest cell viabilities are typically greater than 95%.
The Star-Plus microcarriers are a positive-charge, rigid spherical Animal Protein Free microcarrier and under a variety of environmental conditions, foster quick cell attachment and excellent growth of multiple cell types. Ideal for the incorporation into single-use manufacturing processes, each manufactured lot of Star-Plus microcarriers is performance tested for cell attachment and growth.
Collagen Coated
For anchorage-dependent cell lines, collagen has demonstrated to be an effective promoter of cell attachment and growth. SoloHill® Collagen Coated microcarriers promote rapid attachment and cell growth for a large variety of cell lines.
FACT III
Compared to standard collagen-coated microcarriers, Fast Attachment Collagen Treated (FACT) have improved attachment kinetics and enhance growth performance through the utilization of proprietary surface modification of the collagen layer.