SoloHill® Microcarriers

Animal Protein Coated and Animal Product Free Microcarriers

SoloHill® Microcarriers promote excellent anchorage-dependent cell line attachment and growth and have been used for the production of animal and human health products for over 30 years. Without hydration or pre-swelling needed, microcarriers are easily sterilized for use, and their solid core design simplifies seed train preparation. Available both in animal protein-coated and animal product free, SoloHill® Microcarriers fit into any process and are an excellent choice for scale-up projects where process development timelines and capital investment are limiting.


Microcarriers - Find the Right Solution

SoloHill® Animal Product Free Microcarriers are for researchers working in limited or without animal sources in their processes. Sartorius offers a high performing certified animal-free microcarrier, perfect for researchers who desire to work within a completely serum-free media and animal product free (APF) process.
 

Hillex®

Plastic

Plastic Plus

Star-Plus

Description

Modified Polystyrene

Cross-linked Polystyrene

Cross-linked Polystyrene

Cross-linked Polystyrene

Size Range

160 – 200 µm

90 - 150 & 125 - 212 µm

125 - 212 µm

125 - 212 µm

Specific Gravity

1.090 - 1.150

1.022 - 1.030 & 1.034 - 1.046

1.022 - 1.030

1.020 - 1.035

Area/Gram 

515 cm2

480 & 360 cm2

360 cm2

360 cm2

Surface Charge 

Yes

No

Yes

Yes

Learn More

Learn More

Learn More

Learn More

Animal collagen has proven to be a useful promoter of cell attachment and growth in monolayer cultures of adherent cell lines. SoloHill® animal protein coated microcarriers can be easily moved between collagen-based monolayers to more space-efficient 3D cultures and are ideal for cell attachment kinetics across a wide variety of tested cell lines.
 

Collagen Coated

FACT III (Collagen)

Description 

Cross-linked Polystyrene

Cross-linked Polystyrene

Animal Protein 

Yes - Type 1 porcine collagen coating

Yes – Surface modified Type 1 porcine collagen coating

Size Range 

90 - 150 & 125 - 212 µm

125 - 212 µm

Specific Gravity 

1.022 - 1.030 & 1.034 - 1.046

1.022 - 1.030

Area/Gram 

480 & 360 cm2

360 cm2

Surface Charge

No

Yes

Learn More

Learn More

SoloHill® Microcarrier Applications

Cell culture and tissue culture scientists worldwide have reported success using SoloHill microcarriers. Literature references and researcher feedback conclusively demonstrate flexibility in multiple adherent applications.


Researcher Reported Applications

Cell Line

Description

Star Plus

Plastic & Plastic Plus

Collagen Coated

Charged Collagen Coated

VERO

monkey kidney

x

x

x

CEF

chick embryo fibroblast

x

ST

swine testes

x

BT

bovine turbinate

x

P4RSF

rabbit skin fibroblast

x

MRC-5

human diploid fibroblast

x

KB

human epithelial carcinoma

x

HFF

human foreskin fibroblast

x

x

x

UMSCC

human squamous carcinoma

x

x

x

x

MDBK

Madin-Darby bovine kidney

x

x

CHO

chinese hamster ovary

x

MDCK

Madin-Darby canine kidney

x

hMSC

Human Mesenchymal Stem Cells

x

x


SoloHill® Microcarriers for Therapeutics and Vaccines

SoloHill® microcarriers have been used in the commercial production of both human and animal biopharmaceutical products.


FDA-licensed human therapeutics:

  • Thyroid cancer diagnostic
  • Thyroid cancer therapy
  • Beta interferon – MS indication

USDA-licensed animal vaccines:

  • Equine influenza
  • Canine parvovirus
  • Feline leukemia and many others

SoloHill scientists are actively researching new applications. This research has led to completed development on novel human vaccine protocols.

  • Human influenza vaccine
  • Marek's disease vaccine
Animal Component Free Microcarriers

The cationic amine-modified microporous surface of the Hillex® microcarriers with polystyrene core improves cell attraction and attachment without affecting cell harvest efficiency.


Request a Quote

Compared to porous microcarriers, styrene copolymer microcarriers are constructed from solid non-toxic plastic which prevents absorption of toxic byproducts and makes harvesting simple with cell viabilities typically greater than 95%. The plastic beads allow for easy process transfer out of traditional styrene labware methods such as roller bottles and monolayers.


Request a Quote

Incorporated with an electric charge, the Plastic Plus microcarriers strengthen cell attraction and attachment. Like the non-toxic Plastic microcarriers, Plastic Plus are solidly constructed from styrene copolymer, and harvest cell viabilities are typically greater than 95%.


Request a Quote

The Star-Plus microcarriers are a positive-charge, rigid spherical Animal Protein Free microcarrier and under a variety of environmental conditions, foster quick cell attachment and excellent growth of multiple cell types. Ideal for the incorporation into single-use manufacturing processes, each manufactured lot of Star-Plus microcarriers is performance tested for cell attachment and growth.


Request a Quote

Animal Protein Coated Microcarriers

Collagen Coated

For anchorage-dependent cell lines, collagen has demonstrated to be an effective promoter of cell attachment and growth. SoloHill® Collagen Coated microcarriers promote rapid attachment and cell growth for a large variety of cell lines.


Request a Quote

FACT III

Compared to standard collagen-coated microcarriers, Fast Attachment Collagen Treated (FACT) have improved attachment kinetics and enhance growth performance through the utilization of proprietary surface modification of the collagen layer.


Request a Quote

Request a Quote