Utilizing High-Quality Growth Factors and Cytokines in the Development of iPSC-Derived Hepatic Organoids
Induced pluripotent stem cells (iPSCs) are a flexible source of tissue models for drug discovery, cancer research and disease modeling. Transforming to miniature organs of interest through differentiation to emulate structural and functional functions as an organoid.
Growth factors are crucial for the maintenance of iPSCs; the timing and intensity of their release is responsible for determining the success of developmental and differentiation processes.
Overview
This application note provides a robust and standardized workflow using Sartorius Research Use Only (RUO) Growth Factors and Cytokines in combination with the Incucyte® Live-Cell Analysis System and the iQue® Flow Cytometer for phenotypic characterization and monitoring of grow.
Discover a simplified approach to functional hepatic cells and organoid generation from iPSC drug discovery, development, and toxicity studies.
Document Type: Application Note
Page Count: 12
Read Time: 20 mins
Authors: Daryl Cole, Natasha Lewis, Amber Ward, Jasmine Trigg, Nicola Bevan
- Discover the expanding potential of iPSCs generating various cell types for advanced research
- Common challenges and solutions of organoid development
- Methods to bypass limitations of traditional tissue cultures
- Understand the efficacy of Sartorius RUO Growth Factors and Cytokines as media supplementation for the development and culture of human hepatic organoids
Cover: Utilizing High-Quality Growth Factors and Cytokines in the Development of iPSC-Derived Hepatic Organoids