Mycap® CCX Cell Culture Shake Flask System
Never Open a Flask – Never Go Into a Biosafety Cabinet
The Mycap® CCX cell culture shake flask system features integrated tubing and a specially designed gas exchange cartridge within a single-cap closure, enabling you to grow cells in an incubator and passage them between flasks — without ever opening a vessel or entering a biosafety cabinet. This Sartorius solution is supplied fully assembled with an Erlenmeyer flask, and is gamma-irradiated and ready to use.
Automated Closure System for Cell Culture Expansion
Mycap® CCX enables closed, automatic seed train passaging outside the biosafety cabinet. Integrated with Cubis® II, it supports precise, traceable operations that comply with GMP requirements.
Modernize Your Cell Expansion Process
Mycap® CCX Overview
Mycap® CCX is a closed-system cell culture shake flask with integrated tubing and gas exchange for aseptic cell growth and passaging. Easily culture and transfer cells without opening the flask or using a biosafety cabinet — reducing contamination risk and simplifying your workflow.
Embrace the Closed Phase During Your Seed Train Process
Mycap® CCX Performance
We graphed the average culture doubling times for each flask size for Mycap® CCX flasks and traditional flasks.
Cell growth rates and cell culture doublings are what benchmark successful passages in an expansion process. Based on our results, cell culture doublings between Mycap® CCX and traditional flasks across four passages are equivalent.
Cell Passing With Mycap® CCX and Cubis® II QApp
Mycap® CCX is a single-use seed train solution, designed for seamless integration into manufacturing environments. The Cubis® II software application for Mycap® CCX supports liquid transfer using Watson-Marlow peristaltic pumps. The software automatically calculates the volume to transfer and records the final volume and effective cell density.
Build the Business Case for Mycap® CCX
Go beyond intuition and quantify the impact on your business operations with Mycap® CCX.
The model considers the benefits gained with Mycap® CCX like improved efficiency, reduced environmental monitoring, contamination avoidance, and more.
Enter and adjust variables based on your facility and processes, and the Mycap® CCX Business Case Model will calculate the impact of Mycap® CCX on your operations in simple terms.
Learn More About Mycap® CCX
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Mycap® CCX Validation Tool
Join the movement and begin validation of Mycap® CCX for your operations.
Define your expansion process parameters and critical performance metrics, and the tool creates parts and process maps to simplify your experimental execution.
Record the experiment’s data to instantly generate charts visualizing results, perform statistical analysis, and compare growth data between Mycap® CCX and your existing process.
Frequently Asked Questions
Mycap® CCX is designed as a fully closed-system, GMP-ready seed train solution. Through its integrated weldable tubing, it supports seamless, aseptic fluid transfer from the initial shake flask expansion directly into larger single-use bioreactors, all without exposing the culture to the environment.
Yes. Closed-system solutions like Mycap® CCX feature integrated tubing that is fully compatible with peristaltic pumps. When paired with software like the Cubis® II QApp, you can automate and precisely document media fills and cell passage volumes directly on the benchtop, eliminating the need for a biosafety cabinet.
Yes. Closed-system solutions like Mycap® CCX are provided with thermo-welding tubing in addition to an aspetic connector. By using peristaltic pumps, you can transfer from one closed system to another outside of the biosafety cabinet.
Switching to a closed-system workflow eliminates the need for laminar flow hood scheduling, reduces environmental monitoring costs, and drastically lowers the risk of batch-ruining contamination. This results in streamlined operations, fewer redundant back-up passages, and significant long-term labor and facility savings.
Validation is achieved by testing the system's ability to maintain sterility during passive gas exchange and fluid transfer. Sartorius supports this process by providing a comprehensive validation guide and a dedicated Mycap® CCX Validation Tool, which generates complete parts and process maps to simplify experimental execution and data analysis.
No. Our extensive application testing demonstrates that the Mycap® CCX high surface-area gas exchange cartridge provides passive, unrestricted aeration that yields equivalent or superior cell growth rates and viabilities when compared to traditional, open-cap Erlenmeyer flasks.
Mycap® CCX addresses multiple requirements described by Annex 1:
Closed systems | Article 8.127:
The use of closed systems can reduce the risk of microbial, particle and chemical contamination from the adjacent environment. Closed systems should always be designed to reduce the need for manual manipulations and the associated risks.Single-use systems (SUS) | Articles 8.131:
SUS are those technologies used in manufacture of sterile products which are used as an alternative to reusable equipment. SUS can be individual components or made up of multiple components such as bags, filters, tubing, connectors, valves, storage bottles and sensors. Single use systems should be designed to reduce the need for manipulation and complexity of manual interventions.
250 mL: ≈0.60mL
500 mL: ≈0.60mL
1000 mL: ≈0.60mL
2000 mL: ≈0.90mL
3000 mL: ≈0.90mL
