Mycap® CCX Cell Culture Shake Flask
Never Open a Flask – Never Go Into a Biosafety Cabinet
Mycap® CCX cell culture shake flasks feature integral tubing and a specially designed gas exchange cartridge within a single cap closure, allowing cells to be grown in an incubator and passaged between flasks without ever opening a vessel or entering a biosafety cabinet. This sartorius solution is supplied fully assembled with an Erlenmeyer flask, gamma-irradiated, and ready to use.
Automated Closure System for Cell Culture Expansion
Mycap® CCX enables closed, automatic seed train passaging outside the biosafety cabinet. Integrated with Cubis II, it supports precise, traceable operations that comply with GMP requirements.
Modernize Your Cell Expansion Process
Mycap® CCX Overview
Mycap® CCX is a closed‑system cell culture shake flask with integrated tubing and gas exchange for aseptic cell growth and passaging. Culture and transfer cells without opening the flask or using a biosafety cabinet—reducing contamination risk and simplifying workflows.
Embrace the Closed Phase During Your Seed Train Process
Mycap® CCX Performance
Average culture doubling times for each flask size were graphed. The graph illustrates the comparability of doubling times for Mycap® CCX flasks and traditional flasks.
Successful passages in an expansion process are benchmarked by cell growth rates and cell culture doublings. A comparison of cell culture doublings between Mycap® CCX and traditional flasks across 4 passages were found to be equivalent.
Cell Passing With Mycap® CCX and Cubis® II QApp
Mycap® CCX is a single-use seed train solution, designed for seamless integration into manufacturing environments. The Cubis® II software application for Mycap® CCX facilitates liquid transfer using Watson-Marlow peristaltic pumps. The software automatically calculates the volume to be transferred, records the final volume and effective cell density.
Build the Business Case for Mycap® CCX
Go beyond intuition and quantify the impact on your business operations with Mycap® CCX.
The model considers the benefits gained with Mycap® CCX such as improved efficiency, reduced environmental monitoring, avoidance of contamination, and more.
Enter and adjust variables based on your facility and processes and the Mycap® CCX Business Case Model calculates the impact of Mycap® CCX on your operations in simple terms.
Learn More About Mycap® CCX
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Mycap® CCX Validation Tool
Join the movement and begin validation of Mycap® CCX for your operations.
Define expansion process parameters and critical performance metrics and the Tool creates a complete parts and a process map to make experimental execution simple.
Record the experiment’s data to instantly generate charts visualizing results, perform statistical analysis and compare growth data between Mycap® CCX and the existing process.
Frequently Asked Questions
The Mycap® CCX is designed as a fully closed-system, GMP-ready seed train solution. Through its integral weldable tubing, it allows for seamless, aseptic fluid transfer from the initial shake flask expansion directly into larger single-use bioreactors without exposing the culture to the environment.
Yes. Closed-system solutions like the Mycap® CCX feature integral tubing that is fully compatible with peristaltic pumps. When paired with software like the Cubis® II QApp, operators can automate and precisely document media fills and cell passage volumes directly on the benchtop, completely eliminating the need for a biosafety cabinet.
Yes. Closed-system solutions like the Mycap® CCX are providing with thermo welding tubing an/or aspetic connector - by using peristaltic pumps the operator can transfer from one closed system to another outside of the biosafety cabinet.
Switching to a closed-system workflow eliminates the need for laminar flow hood scheduling, reduces environmental monitoring costs, and drastically lowers the risk of batch-ruining contamination. This results in streamlined operations, fewer redundant backup passages, and significant long-term labor and facility savings.
Validation is achieved by testing the system's ability to maintain sterility during passive gas exchange and fluid transfer. Sartorius supports this process by providing a comprehensive Validation Guide and a dedicated Mycap® CCX Validation Tool, which generates complete parts and process maps to simplify experimental execution and data analysis.
No. Extensive application testing demonstrates that the Mycap® CCX high-surface-area gas exchange cartridge provides passive, unrestricted aeration that yields equivalent or superior cell growth rates and viabilities when compared to traditional, open-cap Erlenmeyer flasks.
From Annex 1 GMP:
- Closed systems | Article 8.127:
The use of closed systems can reduce the risk of microbial, particle and chemical contamination from the adjacent environment. Closed systems should always be designed to reduce the need for manual manipulations and the associated risks. - Single-use systems (SUS) | Articles 8.131:
SUS are those technologies used in manufacture of sterile products which are used as an alternative to reusable equipment. SUS can be individual components or made up of multiple components such as bags, filters, tubing, connectors, valves, storage bottles and sensors. Single use systems should be designed to reduce the need for manipulation and complexity of manual interventions.
Mycap® CCX is the shake flasks solution closure system who reach an equivalent performance face to traditional shake flask cell culture.
- 250mL: ≈0.60mL
- 500mL: ≈0.60mL
- 1000mL: ≈0.60mL
- 2000mL: ≈0.90mL
- 3000mL: ≈0.90mL
