Biologics & Small Molecules Research

Biologics and Small Molecules Research

  • Measure target binding affinity and kinetics of purified and non-purified biological molecules 
  • Rapidly perform DOEs to screen optimal assay conditions

The development of biologics-based drugs is an expensive and lengthy process. During early discovery, researchers must select multiple lead candidates, confirm mechanisms of action (MOA) against the target and investigate optimal conditions for production and functional activity before looking into their downstream critical quality attributes (CQAs). The Octet® system provides unmatched ease-of-use and throughput capability in research and assay development for screening and characterization purposes.

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Featured Applications

Fc Receptor Binding Assays diagram

Fc Receptor Binding Assays on the Octet® BLI Platform

The selection of desired antibody-based therapeutics is often based on their binding properties, including binding to FcγRs. Antibodies are sometimes engineered to achieve desired FcγRs binding properties, as their binding can greatly impact their safety and efficacy to both the target and to FcγRs. 

  • Octet® BLI systems offer a high throughput, sensitive solution for Fc receptor binding analysis
  • A variety of assay-ready biosensor surfaces are available and allow for flexibility and rapid optimization of assays

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Small Molecule and Peptide Binding Kinetics

The discovery of small molecule and peptide lead molecules can stem from many sources, including fragment screening, high throughput screening and de novo structural design. Determining and evaluating the affinity of small molecule binding to a therapeutic target is a significant component of the drug discovery process and lead optimization. The hit-to-lead and lead optimization processes are essential to accurately determine biological potency in vitro so structure-activity relationships (SARs) can be used for efficient structural design. Learn how Octet® R series as well as Octet® RH16, Octet® RH96 and Octet® SF3 instruments can be used to characterize small molecule and peptide binding.

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High Throughput Phage Display Screening

Phage display is a technique for enabling the study of protein, peptide or DNA interactions with a target protein. This technology enables discovery of high-affinity binders by using bacteriophages to present a target protein on the exterior of the viral coat (while containing the DNA encoding the target protein inside the viral coat). Resulting phages can be screened for binding against a library of peptides or proteins in a high throughput fashion. 

Due to its high throughput design, the Octet® BLI system is routinely used as a secondary screening platform for Fab fragments and non-antibody ligands derived from phage display libraries. Such a screen can provide affinity ranking data and estimates of association and dissociation constants for primary hits.

Characterize Irreversible Inhibitors and Measure Commitment to Covalency

The majority of small molecule inhibitor assays tested with label-free, real-time biosensor technologies are reversible interactions, characterized by commonly used kinetic rate models. However, a significant fraction of therapeutic enzyme inhibitors on the market function through covalent modification of the target.

The Octet® SF3 system is a SPR platform that can be used with regenerable Streptavidin biosensors to reversibly capture protein targets and quantify the efficiency of covalent inhibitors binding to the target. The irreversible inhibitor applications method can be used to determine inhibitor compound's commitment to covalency (Cc) as a metric for irreversible inhibitors.

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Featured Resources

Octet® BLI Workflows in Small-Molecule Interaction Discovery and Characterization
Application Guide

Small-Molecule Interaction Discovery and Characterization

With Octet® BLI Workflows

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A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

Octet® Label-Free Biosensor Analysis

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Influenza Vaccine Titre Determination Bio-Layer Interferometry (BLI)

Fast, accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and correctly sca...

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Application Guide

Recent Insights into Covid-19 Binding Epitopes

Researchers are using the Octet® system to understand the virus's binding mechanisms as a first step in the development of a vaccine

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Cover: Characterizing Membrane Protein Interactions by Bio-Layer Interferometry
Application Guide

Characterizing Membrane Protein Interactions

By Octet® Bio-Layer Interferometry (BLI) Platforms

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Label-Free Detection technologies, key considerations and applications cover

Label-Free Detection Technologies: Key Considerations and Applications

Technologies, Key Considerations, and Applications

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Application Note cover
Application Guide

Label-Free Technologies

For Accurate Determination of Kinetics and Affinity Rate Constants

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White Paper

Writing Grant Proposals for Biomolecular Interactions Research

Tips collected over the years from customers successful with their equipment grant proposals.

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Instant Access

Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles
Application Note

Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles

PDF | 929.6 KB
Optimizing Protein-Protein and Protein-Small Molecule Kinetics Assays
Application Note

Optimizing Protein-Protein and Protein-Small Molecule Kinetics Assays

PDF | 950.7 KB
Application Note

Kinetic Curvature: Assessment of Small Molecule Kinetics and Affinity Using OneStep® Injections in SPR Screening

PDF | 289.9 KB
Small Molecule Binding Kinetics
Technical Note

Small Molecule Binding Kinetics

PDF | 2.7 MB
A Fast and High Precision Influenza Vaccine Potency Assay
Application Note

A Fast and High Precision Influenza Vaccine Potency Assay

PDF | 687.7 KB
Instant Determination of Protein Presence Using Octet® N1 System
Application Note

Instant Determination of Protein Presence Using Octet® N1 System

PDF | 762.6 KB
app note cover
Application Note

Reducing Variability in Small Molecule Screening and Kinetics Applications

PDF | 1.4 MB
Octet® SF3 SPR

Octet® SF3 SPR 

Generate high-quality kinetics and affinity data in a fraction of the time compared to standard multi-cycle kinetics using the Octet® SF3.

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Octet® BLI rapidly determines recombinant protein concentration as a control parameter in cell line development.

96-Channel Ultra High Throughput Octet System: Octet® RH96

Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed

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Octet RH16 ultra high throughput label free detection system

16-Channel High Throughput Octet System: Octet® RH16

Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements

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