Biologics & Small Molecules Research

Biologics & Small Molecules Research

Biologics Research and Development

  • Measure target binding affinity and kinetics of purified and non-purified biological molecules
  • Rapidly perform DOEs to screen optimal assay conditions

The development of Biologics based drugs is an expensive and lengthy process. Early discovery requires researchers to select multiple lead candidates, confirm their mechanisms of action (MOA) against the target, and investigate optimal conditions for their production and functional activity before looking into their downstream critical quality attributes. The Octet® system provides unmatched ease-of-use and throughput capability in research and assay development for screening and characterization purposes.

Features

Fc Receptor Binding Assays on the Octet® System

The selection of desired antibody-based therapeutics is often based on their binding properties, including binding to FcγRs. Antibodies are sometimes engineered to achieve desired FcγRs binding properties, as their binding can greatly impact their safety and efficacy to both the target and to FcγRs.

  • Octet® systems offer a high-throughput and sensitive solution for Fc receptor binding analysis
  • A variety of assay-ready biosensor surfaces are available and allow for flexibility and rapid optimization of assays

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Small Molecule and Peptide Binding Kinetics

The discovery of small molecule and peptide lead molecules can stem from many sources including, fragment screening, high throughput screening and de novo structural design, amongst others. Determining and evaluating the affinity of small molecule binding to a therapeutic target is a significant component of the drug discovery process and lead optimization. The hit-to-lead and lead optimization processes are essential to accurately determine biological potency in vitro so that structure-activity relationships (SARs) can be used for efficient structural design. Learn how Octet® R Series of Systems, Octet® RH16, Octet® RH96 and Pioneer SPR platforms can be used to characterize small molecule and peptide binding.

High Throughput Phage Display Screening

Phage display is a technique to enable the study of protein, peptide, or DNA interactions with a target protein. This technology enables the discovery of high-affinity binders by using bacteriophages to present a target protein on the exterior of the viral coat, while containing the DNA encoding the target protein inside the viral coat. The resulting phages can be screened for binding against a library of peptides or proteins in a high throughput fashion.

Due to its high throughput design, the Octet® system is routinely used as a secondary screening platform for Fab fragments and non-antibody ligands derived from phage display libraries. An Octet® system screen can provide affinity ranking data and estimates of association and dissociation constants for primary hits.

Characterize Irreversible Inhibitors and Measure Commitment to Covalency

The majority of small molecule inhibitor assays tested with label-free, real-time biosensor technologies are reversible interactions, characterized by commonly used kinetic rate models. However, a significant fraction of therapeutic enzyme inhibitors on the market functions through covalent modification of the target.

The Pioneer FE system is a SPR platform that can be used with regenerable Streptavidin biosensors to reversibly capture protein targets and quantify the efficiency of covalent inhibitors binding to the target. The Pioneer FE system's irreversible inhibitor applications method can be used to determine inhibitor compound's commitment to covalency (Cc) as a metric for irreversible inhibitors.

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Resources

Influenza Vaccine Titre Determination Bio-Layer Interferometry (BLI)

Fast, accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and...

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Recent Insights into Covid-19 Binding Epitopes

Bio-Layer Interferometry (BLI) technology is helping scientists around the world learn more about the recent Coronavirus (COVID-19) outbreak. In...

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Cover: Characterizing Membrane Protein Interactions by Bio-Layer Interferometry

Characterizing Membrane Protein Interactions by Bio-Layer...

Example studies on how the Octet® system can be used to analyze membrane protein interactions, even allowing the use of unpurified crude matrices...

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Label-Free Detection Technologies: Key Considerations and Applications cover

Label-Free Detection Technologies: Key Considerations and Applications

Binding interactions in biological samples are dynamic and driven by changes to the environment. The techniques used to characterize these...

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Application Note cover

Label-Free Technologies for Accurate Determination of Affinity and...

From target molecule identification to lead selection and optimization, equilibrium affinity and kinetics rate constants are critical parameters...

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Writing Grant Proposals for Biomolecular Interactions Research

Grant writing can be a grind. You know how and why the funding is needed, but communicating that successfully sometimes seems like an art.

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A Fast and High Precision Influenza Vaccine Potency Assay cover

A Fast and High Precision Influenza Vaccine Potency Assay

Mall molecule and peptide therapeutic drugs are highly sought after in most areas of disease research due to their desirable pharmacological...

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Instant Determination of Protein Presence Using BLItz System

Often, precious time is lost during protein expression and bioreactor monitoring when simply checking for the presence or absence of the target...

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Kinetics of Irreversible Inhibitors on the Pioneer FE System

The principal role of assay groups in drug discovery is to provide reliable methods, analysis, and data for confident decision-making about...

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Kinetic Analysis of AB Binding to Membrane Protein on Captured...

Membrane proteins govern the majority of input and output signals of cells and represent the largest class of pharmaceutical drug targets, making...

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Reducing Variability in Small Molecule Screening and Kinetics...

Minimizing the variability of background signals is a key parameter to the success of demanding applications such as small molecule analysis

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Pioneer FE SPR System

Pioneer FE SPR System

Make Decisions Earlier in Fragment Screens with the Pioneer FE

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Pioneer SPR System

Complete solution for biomolecular interaction analysis with next generation SPR injections

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96-Channel Ultra High Throughput Octet System: Octet® RH96

Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed

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16-Channel High Throughput Octet System: Octet® RH16

The Octet® RH16 16-channel instrument is ideal for high-throughput applications that demand high sensitivity and low sample volume requirements

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Octet RED96e

8-Channel Octet System: Octet RED96e

The 8-channel Octet systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1.5 hours.

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