Biologics & Small Molecules Research

Biologics & Small Molecules Research

Biologics Research and Development

  • Measure binding affinity of purified and non-purified biological molecules
  • Rapidly perform DOEs to screen optimal assay conditions

The development of Biologics based drugs is an expensive and lengthy process. Early discovery requires researchers to select multiple lead candidates, confirm their mechanisms of action (MOA) against target, and investigate optimal conditions for their production and functional activity before looking into their downstream critical quality attributes. The Octet system provides unmatched ease-of-use and throughput capability in research and assay development for screening and characterization purposes.


ELISA Conversion on the Octet System

While commonplace, ELISA is recognized for variability in results, susceptibility to human error, labor-intensive workflows, and slow time-to-results. Faster times-to-results, better consistency, and less human intervention are the prerequisites for next generation of high throughput laboratory operation. Octet systems are configured to allow researchers to:

  • Transfer your ELISA assay onto the Octet system for real-time analysis
  • Increase precision over manual ELISA in direct quantitation assays
  • Amplify response signals in multistep quantitation assay to increase quantitation sensitivity

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High Throughput Phage Display Screening

Phage display is a technique to enable the study of protein, peptide or DNA interactions with a target protein. This molecular tool enables the discovery of high-affinity binders by using bacteriophages to present a target protein on the exterior of the viral coat, while containing the DNA encoding the target protein inside the viral coat. The resulting displaying phages can be screened for binding against a library of peptides or proteins in a high throughput fashion.

Due to its high throughput design, the Octet system is routinely used as a secondary screening platform for Fab fragments and non-antibody ligands derived from phage display libraries. Using immobilized antigen, an Octet system screen can provide affinity ranking data and estimates of association and dissociation constants for each primary hit.

Virus Titer and Vaccine R&D on the Octet System

Bio-analytical tools that aid in the speedy development of new vaccines that can be used to combat the spread of pandemics such as Corona virus (Covid19) are desirable in every cell line development, process development and manufacturing labs. A method for the rapid titer of viruses and for the basic research required for understanding the mechanism of action of molecules involved in a pandemic is critical to the rapid development of a vaccine against the virus.

The Octet instrument is aptly designed to provide the throughput needed to quickly screen antibodies and other biological molecules involved in various virus-related diseases for binding specificity and affinity, epitope binning, neutralization and competition studies and for virus titer. The platform is routinely used for studies and titer determination of viruses and virus like particles including Influenza, Adeno Associated Virus (AAV), Dengue and other viruses and is providing much needed insights in the fight against the Coronavirus.

  • High throughput and rapid potency testing
  • Quantitate or perform rapid virus affinity characterization
  • Develop stability indicating methods
  • Study and test intact virus samples

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Characterize Irreversible Inhibitors and Measure Commitment to Covalency

The majority of small molecule inhibitor assays tested with label-free, real-time biosensor technologies are reversible interactions, characterized by commonly used kinetic rate models. However, a significant fraction of therapeutic enzyme inhibitors on the market functions through covalent modification of the target.

The Pioneer FE system is a SPR platform that can be used with regenerable Streptavidin biosensors to reversibly capture protein targets and quantify the efficiency of covalent inhibitors binding to the target. The Pioneer FE system's irreversible inhibitor applications method can be used to determine inhibitor compound's commitment to covalency (Cc) as a metric for irreversible inhibitors.

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Influenza Vaccine Titre Determination Bio-Layer Interferometry (BLI)

Fast, accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and...

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Recent Insights into Covid-19 Binding Epitorpes

Bio-Layer Interferometry (BLI) technology is helping scientists around the world learn more about the recent Coronavirus (COVID-19) outbreak. In...

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Cover: Characterizing Membrane Protein Interactions by Bio-Layer Interferometry

Characterizing Membrane Protein Interactions by Bio-Layer...

Example studies on how the Octet® system can be used to analyze membrane protein interactions, even allowing the use of unpurified crude matrices...

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Label-Free Detection Technologies: Key Considerations and Applications

Binding interactions in biological samples are dynamic and driven by changes to the environment. The techniques used to characterize these...

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Label-free technologies for accurate determination of affinity and kinetics rate constants

Label-Free Technologies for Accurate Determination of Affinity and...

From target molecule identification to lead selection and optimization, equilibrium affinity and kinetics rate constants are critical parameters...

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Writing Grant Proposals for Biomolecular Interactions Research

Grant writing can be a grind. You know how and why the funding is needed, but communicating that successfully sometimes seems like an art.

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A Fast and High Precision Influenza Vaccine Potency Assay

Mall molecule and peptide therapeutic drugs are highly sought after in most areas of disease research due to their desirable pharmacological...

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Instant Determination of Protein Presence Using BLItz System

Often, precious time is lost during protein expression and bioreactor monitoring when simply checking for the presence or absence of the target...

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Kinetics of Irreversible Inhibitors on the Pioneer FE System

The principal role of assay groups in drug discovery is to provide reliable methods, analysis, and data for confident decision-making about...

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Kinetic Analysis of AB Binding to Membrane Protein on Captured...

Membrane proteins govern the majority of input and output signals of cells and represent the largest class of pharmaceutical drug targets, making...

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Reducing Variability in Small Molecule Screening and Kinetics...

Minimizing the variability of background signals is a key parameter to the success of demanding applications such as small molecule analysis

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Pioneer FE SPR System

Pioneer FE SPR System

Make Decisions Earlier in Fragment Screens with the Pioneer FE

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Pioneer SPR System

Complete solution for biomolecular interaction analysis with next generation SPR injections

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Octet HTX

96-Channel Ultra High Throughput Octet System: Octet HTX

Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed

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Octet RED384

16-Channel High Throughput Octet System: Octet RED384

The Octet RED384 16-channel instrument provides analytical performance similar to the 8- channel Octet RED96 system, but with higher levels of...

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Octet RED96e

8-Channel Octet System: Octet RED96e

The 8-channel Octet systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1.5 hours.

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