Affinity & Kinetic Characterization illustration

Affinity & Kinetic Characterization

Label-Free Affinity and Kinetic Characterization

Establishing an in-depth understanding of binding interactions between biological or small molecules is vital to elucidating ligand receptor binding mechanisms and stability during the development of drug molecules. Drugs that exhibit similar affinities could exhibit diverse binding mechanisms and kinetics. Hence, the evaluation of affinity constants in conjunction with association and dissociation rates provides crucial information for lead selection. Octet® BLI assays have become increasingly valuable for affinity and kinetic characterization of biomolecules.

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Featured Applications

Large Molecules Kinetics Characterization

The Octet® BLI platform accurately measures kinetic constants by bringing the detection surface directly to the sample, eliminating the need for microfluidics. This unique approach using label-free, real-time analysis streamlines laboratory workflows and expedites assay development. The unique platform allows direct measurement from crude samples while minimizing instrument maintenance. It is extremely versatile in large molecules kinetics characterization and enables rapid assay optimization to allow the analysis of molecules ranging from recombinant proteins and antibodies to viruses and nanoparticles.

Fc Receptor Binding Assays diagram

Fc Receptor Binding Assays on the Octet® BLI Platform 

The selection of desired antibody-based therapeutics is often based on binding properties, including binding to FcγRs. Antibodies are sometimes engineered to achieve desired FcγRs binding properties, as their binding can greatly impact safety and efficacy to both the target and to FcγRs. 

  • Octet® BLI systems offer a high throughput, sensitive solution for Fc receptor-binding analysis
  • A variety of assay-ready biosensor surfaces are available and allow for flexibility and rapid optimization of assays

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Small Molecule and Peptide Binding Kinetics

The discovery of small molecule and peptide lead molecules can stem from many sources, including fragment screening, high throughput screening and de novo structural design. Determining and evaluating the affinity of small molecule binding to a therapeutic target is a significant component of the drug discovery process and lead optimization. The hit-to-lead and lead optimization processes are essential for accurate determination of biological potency in vitro so structure-activity relationships (SARs) can be used for efficient structural design. Learn how the Octet® R series as well as Octet® RH16, Octet® RH96 and Octet® SF3 instruments can be used to characterize small molecule and peptide binding.

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High-affinity Interactions

Target binding characterization is an essential analytical step for selecting high-affinity and specific biologics, regardless of molecule type. The characterization of biological molecules with relatively high-affinity target binding (KD <1 nM) is often quite challenging due to the need for high-sensitivity analytical platforms capable of detecting slight changes in responses. The Octet® SF3 instrument is a high-sensitivity instrument that utilizes next generation SPR injections to improve the efficiency of the characterization process by determining kinetics and affinity in a single step. The OneStep® and NeXtStep™ Gradient Injection technologies featured on the Octet® SF3 instrument dramatically increases the speed of affinity characterization while maintaining accuracy and high confidence in results.

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Featured Resources

Application Note

Protein Domain Mapping Chemokines & SARS-CoV-2 Nucleocapsid

This application note discusses and characterizes of the interaction between the SARS-CoV-2 Nucleocapsid (N) protein and human chemokines.

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A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

Octet® Label-Free Biosensor Analysis

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Integrating Octet® BLI into Early Antibody Discovery Workflows

Improve the overall drug discovery process to increase the success rate of preclinical candidates

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Analysis of Fc-Gamma Receptor-IgG Interactions on the Octet® Platform
Application Note

Analysis of Fc Gamma Receptor IgG Interactions on Octet® Platform

Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.

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Analysis of FcRn-Antibody Interactions on the Octet® Platform
Application Note

Analysis of FcRn-Antibody Interactions on the Octet® Platform

Assay design recommendations and best practices for producing the highest quality of kinetic data.

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White Paper

Streamlining Affinity Analysis for Accelerated Lead Screening

Antibody and other protein therapeutics are a major focus in drug discovery pipelines today

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Cover: Characterizing Membrane Protein Interactions by Bio-Layer Interferometry
Application Guide

Characterizing Membrane Protein Interactions

By Octet® Bio-Layer Interferometry (BLI) Platforms

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Application Note cover
Application Guide

Label-Free Technologies

For Accurate Determination of Kinetics and Affinity Rate Constants

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Instant Access

Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles
Application Note

Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles

PDF | 929.6 KB
SPR Fragment based drug discovery
Application Note

Fragment-Based Drug Discovery (FBDD) Using Octet® SPR OneStep® and NeXtStep™ Injections

PDF | 5.1 MB
app note cover
Application Note

Reducing Variability in Small Molecule Screening and Kinetics Applications

PDF | 1.4 MB
app note cover
Application Note

Generating Reliable Kinetic Data For Protein Ligand Interactions

PDF | 130.8 KB
Small Molecule Binding Kinetics
Technical Note

Small Molecule Binding Kinetics

PDF | 2.7 MB
application note thumbnail
Application Note

Kinetics Determination of High Affinity Molecular Interactions Using OneStep® Injections

PDF | 212.2 KB
Octet® SF3 SPR

Octet® SF3 SPR 

Generate high-quality kinetics and affinity data in a fraction of the time compared to standard multi-cycle kinetics using the Octet® SF3.

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Octet® BLI rapidly determines recombinant protein concentration as a control parameter in cell line development.

96-Channel Ultra High Throughput Octet System: Octet® RH96

Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed

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Octet RH16 ultra high throughput label free detection system

16-Channel High Throughput Octet System: Octet® RH16

Ideal for high-throughput applications that demand high sensitivity and low sample volume requirements

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