Affinity & Kinetic Characterization

Label-Free Affinity & Kinetic Characterization

Establishing an in-depth understanding of binding interactions between biological or small molecules is vital to elucidating ligand-receptor binding mechanisms and stability during the development of drug molecules. Drugs that exhibit similar affinities could exhibit diverse binding mechanisms and kinetics.  Hence, the evaluation of affinity constants in conjunction with the association and dissociation rates provides crucial information for lead selection. Octet® assays have become increasingly valuable for affinity and kinetic characterization of biomolecules providing detailed binding profiles of biomolecules.

Featured Applications

Large Molecules Kinetics Characterization

The Octet® family of instruments accurately measure kinetic constants by bringing the detection surface directly to the sample, eliminating the need for microfluidics. This unique approach utilizing label-free, real-time analysis streamlines laboratory workflows and expedites assay development. It allows direct measurement from crude samples while minimizing instrument maintenance. It is extremely versatile in large molecules kinetics characterization and enables rapid assay optimization to allow for the analysis of molecules ranging from recombinant proteins and antibodies to viruses and nanoparticles.

Fc Receptor Binding Assays on the Octet® System

The selection of desired antibody-based therapeutics is often based on their binding properties, including binding to FcγRs. Antibodies are sometimes engineered to achieve desired FcγRs binding properties, as their binding can greatly impact their safety and efficacy to both the target and to FcγRs.

  • Octet® systems offer a high- throughput and sensitive solution for Fc receptor binding analysis
  • A variety of assay-ready biosensor surfaces are available and allow for flexibility and rapid optimization of assays

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Small Molecule and Peptide Binding Kinetics

The discovery of small molecule and peptide lead molecules can stem from many sources including, fragment screening, high throughput screening and de novo structural design amongst others. Determining and evaluating the affinity of small molecule binding to a therapeutic target is a significant component of the drug discovery process and lead optimization. The hit-to-lead and lead optimization process are essential to accurately determine biological potency in vitro so that structure-activity relationships (SARs) can be used for efficient structural design. Learn how  Octet® R Series of Systems, Octet® RH16, Octet® RH96 and Pioneer SPR platforms can be used to characterize small molecule and peptide binding.

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High-affinity Interactions

Target binding characterization is an essential analytical step for selecting high affinity and highly specific biologics regardless of the types of molecules The characterization of biological molecules with relatively high-affinity target binding (KD <1 nM) is often quite challenging due to the need for high-sensitivity analytical platforms capable of detecting slight changes in responses. The Pioneer SPR is a high sensitivity instrument that utilizes next generation SPR injections to improve the efficiency of the characterization process by determining the kinetics and affinity in a single step. The next-generation OneStep gradient injection featured on the Pioneer platform dramatically increases the speed of affinity characterization while maintaining accuracy and high confidence in results.

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Resources

Analysis of Fc Gamma Receptor IgG Interactions on Octet Platform

Whether you're assessing FcRn-Antibody or FcγR-IgG binding interactions, you need a high throughput, versatile, and easy to use solution.

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Analysis of FcRn-Antibody Interactions on Octet Platform

Assay design recommendations and best practices for producing the highest quality of kinetic data.

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Streamlining Affinity Analysis for Accelerated Lead Screening

Antibody and other protein therapeutics are a major focus in drug discovery pipelines today. The overall process for developing protein...

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Kinetics of Irreversible Inhibitors on the Pioneer FE System

The principal role of assay groups in drug discovery is to provide reliable methods, analysis, and data for confident decision-making about...

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Kinetic Analysis of AB Binding to Membrane Protein on Captured...

Membrane proteins govern the majority of input and output signals of cells and represent the largest class of pharmaceutical drug targets, making...

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Generating Reliable Kinetic Data for Protein-Ligand Interactions

The Community Structure Activity Resource (CSAR, www.csardock.org) group is developing a database of high quality protein-ligand structures and...

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Reducing Variability in Small Molecule Screening and Kinetics...

Minimizing the variability of background signals is a key parameter to the success of demanding applications such as small molecule analysis

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Cover: Characterizing Membrane Protein Interactions by Bio-Layer Interferometry

Characterizing Membrane Protein Interactions by Bio-Layer...

Example studies on how the Octet® system can be used to analyze membrane protein interactions, even allowing the use of unpurified crude matrices...

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Application Note cover

Label-Free Technologies for Accurate Determination of Affinity and...

From target molecule identification to lead selection and optimization, equilibrium affinity and kinetics rate constants are critical parameters...

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Pioneer FE SPR System

Pioneer FE SPR System

Make Decisions Earlier in Fragment Screens with the Pioneer FE

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Pioneer SPR System

Complete solution for biomolecular interaction analysis with next generation SPR injections

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96-Channel Ultra High Throughput Octet System: Octet® RH96

Monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed

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16-Channel High Throughput Octet System: Octet® RH16

The Octet® RH16 16-channel instrument is ideal for high-throughput applications that demand high sensitivity and low sample volume requirements

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Octet RED96e

8-Channel Octet System: Octet RED96e

The 8-channel Octet systems perform quantitation of 96 samples in 32 minutes and kinetic screening of 64 samples in 1.5 hours.

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