Chemotatic Migration and Invasion Assays for Live-Cell Analysis

Chemotatic Migration and Invasion Assays for Live-Cell Analysis

Use the Incucyte® Live-Cell Analysis System to study chemotactic cell migration across 2D substrates and directional cell invasion through 3D biomatrix gels.

  • Visualize chemotaxis in real time with fully automated analysis
  • Use the Incucyte® Clearview 96-well Plate for highly reproducible 96-well kinetic assays
  • Low cell usage ideal for rare, expensive and primary cell populations
  • Chemotactic gradients are stable and maintained over 72 hours

Chemotactic invasion through 3D biomatrix gels

  • Real time imaging and quantification of cell invasion through 3D biomatrix gels
  • Assess metastatic potential and define the effect of treatments on invasive phenotype.
  • Explore differential biology of chemotactic cell migration and invasion in the same plate.

Learn more

Chemotactic transendothelial migration of leukocytes

  • Monitor and quantify directional leukocyte migration across an endothelial monolayer in real time
  • Visualize endothelial monolayer integrity and leukocyte diapedesis
  • Investigate the biological effects of inhibitors and neutralizing antibodies

Learn more

4th Edition! Live-Cell Analysis Handbook

A guide to real-time live-cell imaging & analysis

Download Your Copy

Application Note:

Gain additional insights into the mechanism of immune cell killing by combining live cell analysis and flow cytometry into a single workflow

Download Your Copy

A Science Oncology Supplement:

Interrogating the molecular basis of cell migration and metastasis

Download Your Copy
Commonly Used Approaches

In vitro assays for cell migration and chemotaxis are necessary for both basic-research and drug discovery. The table below shows capabilities and challenges of some common approaches.

Existing Boyden ChambersMicrofluidic Devices

Incucyte® Chemotaxis Cell Migration and Invasion

Chemotactic migration  

 

Migration in the absence of chemotactic gradients  

 

Low cell usage (<5,000 per well)  

 

Suitable for non-adherent cells  

 

Migration across a surface  

 

Invasion through a 3D gel matrix

 

 

 

Integrated quantitation

 

 

 

Integrated cell observation

 

 

 

Precision, reproducibility

 

 

 

96-well throughput or higher

 

 

 

Kinetic read-out

 

 

 

Label-free

 

 

 

Workflow: set up and walk away

 

 

 


Request More Information

What other areas are you interested in? (select all that apply)

Request a Demo, Literature, or More Information