Sartobind® Q 96-Well Plates
Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.
- Catalog No.:
*Custom/bulk order quotes are provided within 72 hours of request.
Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.
Applications virus and VLP capture:
As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.
- High speed purification at 5 - 30 bed volumes per minute
- No size exclusion effects for large proteins an viruses / VLPs
- >10fold higher binding capacities compared to conventional resins
Applications flow-through polishing:
Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.
Benefits flow-through polishing:
- Virus clearance > 6 log
- DNA below detection limit
- Host cell protein removal > 99 %
- Endotoxin removal > 5 log
- Save 80 % of column costs
Easy to use:
- Plug and play devices from 0.08 mL to 5 L
- Can be used like a filter
- Flow rate up to 30 bed volumes/min
- Re-usable or disposable
Bed Height0.8 mm
Dimensions WxDxH128 × 85.5 × 25 mm
Membrane Area0.7 cm²
Materials of Construction
Housing MaterialPolypropylene (PP)
Material 8-stripPolypropylene (PP)
Material Holding FramePolystyrene
Materials of ConstructionPolyester
Membrane MaterialStabilized reinforced cellulose
Maximum Operating Pressure35 kPa
pH StabilitypH 2 – 14 (short term)
Bed Volume19 µL/well
Ligand Density2 – 5 µeq/cm²
Pore Size3 – 5 µm
Reference ProteinBovine serum albumin (BSA)
Typical Dynamic Binding Capacity 10% per unit0.55 mg/well
Membrane Adsorber Format96-Well Plates
Membrane TypeStrong anion exchange membrane
TypeMembrane adsorber (96-well plate)
What you receive
- 1 operating instruction
- 10 plates
The Vac96 vacuum manifold has been designed specifically for use with Sartobind® 96 multiwell plates. The long drip nozzles on the outlet eliminate cross-talk between flow channels.