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Sartobind® Phenyl 150 mL

Sartobind® Phenyl accomplishes polishing and capturing ten times faster than do conventional HIC resins. The membrane shows comparable, high binding capacity, without size exclusion effects, for proteins in a range of 20 mg mAb/mL membrane, as well as excellent resolution and impurity reduction.

Item no.:  96HICP42E9BFF

Availability:  On Request


Hydrophobic interaction chromatography (HIC) separates and purifies biomolecules based on differences in their hydrophobicity. The phenyl membrane adsorber follows the same rules known from the conventional hydrophobic interaction chromatography. Due to the large pore size, membrane adsorbers show excellent flow properties. There is almost no diffusion limitation of mass transport compared with conventional bead chromatography. Buffers with high concentrations of salt promote the adsorption of proteins on the hydrophobic membrane matrix. Proteins are eluted by decreasing the salt concentration in the elution buffer.

The salt dependence of the membrane is comparable to that of columns with ammonium sulfate, sodium chloride and sodium citrate.


HIC is a powerful method for differentiating target protein and hydrophobic contaminants. Large proteins bind more strongly than do small ones and aggregates even considerably better than monomers due to multipoint attachment.

Buffer with a high ionic strength, usually ammonium sulfate, is initially added to the protein solution. Salt ions in this solution reduce the solvation of solutes in the sample. As solvation decreases, hydrophobic regions that become exposed are adsorbed by the hydrophobic membrane matrix. In the HIC membrane, flexible cellulose is used as a backbone for the phenyl ligand. The membrane can be regenerated by using either water or alcohol.

The HIC membrane is used for the removal of:

  • Aggregates
  • Host cell proteins
  • Viruses
  • Endotoxins
  • Lipids, dyes, detergents and anti foam agents
  • Leached chromatography ligands

The Phenyl membrane can be used also for binding large proteins such as:

  • Monoclonal antibodies
  • Conjugated vaccines, viruses and phages
  • Oligonucleotides


HIC membranes offer the following advantages over conventional HIC columns:

  • Excellent reduction of aggregates
  • 85 % buffer savings
  • 75 % of time saved

Easy to use:

  • Plug and play devices from 0.08 ml to 5 l
  • Can be used like a filter
  • Flow rate up to 5 bed volumes/min
  • Re-usable >100 cycles or disposable

Application Note

Phenyl Membrane Adsorber for Bioprocessing - Sartobind ® Hydrophobic Interaction Membrane Chromatography
en 2.2 MB
Single-Use Membrane Chromatography in ADC Production
en 1.6 MB


Sartobind® Membrane Chromatography
en 3.5 MB


Sartobind® Phenyl, 8 mm bed height
en 1.1 MB


Sartobind® Selection guide
en 1.0 MB

User Manual

Operating Instructions - Sartobind® Phenyl - Void Volume Optimized Capsules and Cassettes With 8 mm Bed Height
en 2.2 MB

Connections (Physical)

  • Connectors
    Sanitary ½ – ¾"


  • Bed Height
    8 mm
  • Membrane Area
    0.55 m²

Materials of Construction

  • Housing Material
    Polypropylene (PP)
  • Materials of Construction
    • Polypropylene, polyester
    • polyester
  • Membrane Material
    Stabilised reinforced cellulose

Operating Conditions

  • Maximum Operating Pressure
    0.4 MPa
  • Maximum Venting Pressure
    0.5 bar, 0.05 MPa, 7 psi
  • Recommended Flow Rate
    0.75 L/min
  • pH Stability
    pH 2 – 14 (short term), pH 3 – 13 (long term)

Physicochemical Information

  • Bed Volume
    150 mL
  • Ligand
  • Ligand Density
    3 µeq/cm²
  • Pore Size
    3 – 5 µm
  • Reference Protein
    Gamma globulin from bovine blood
  • Thermal Stability
    For Sartobind phenyl devices, pretreatment with sodium hydroxide is recommended instead of autoclaving.
  • Typical Dynamic Binding Capacity 10% per unit
    2.2 g
  • Void Volume
    200 mL

Product Information

  • Membrane Adsorber Format
  • Membrane Type
    Hydrophobic interaction chromatography membrane (HIC)
  • Type
    Membrane adsorber
  • What you receive
    • 1 capsule
    • 1 operating instruction
    • 1 certificate
MidiCaps® Holder
MidiCaps® Holder
Item no.: 5ZGLG-0007

The Sartorius Stedim Biotech MidiCaps® holder has been specially designed to integrate MidiCaps® into biopharmaceutical processes.