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HA Ultrogel® - 500 mL Hydroxyapatite Chromatography Resin

HA Ultrogel® is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows mixed mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. A wide variety of applications include separation of proteins, peptides and nucleic acids, from laboratory to production scale.

Item no.:  24775-017

   

HA Ultrogel® resin is a hydroxyapatite agarose composite resin for the separation of biomolecules from research and to manufacturing scale. Hydroxyapatite chromatography is considered to be a “pseudo-affinity” chromatography, or “mixed-mode” ion exchange. It has proven to be an effective purification mechanism in a variety of processes, providing biomolecule selectivity complementary to more traditional ion exchange or hydrophobic interaction techniques.

 

Features and Benefits

- Unique selectivity and separation mechanism, complementary to traditional IEX and HIC
- Protein purification in neutral, non-denaturating conditions
- High porosity, easy cleaning

 

Applications

- Purification of human serum proteins
- Purification of basic proteins, of phosphate-dependent proteins and enzymes, and of DNA-dependent enzymes
- Vaccine purification processes, e.g. Bordetella pertussis toxin
- Separation of plant proteins such as  lectins, glycoproteins, glycosidases, phospholipidases, sulfohydrolases, sphingomyelinases, transferases, trehalases and kinases

 

Available Formats for HA Ultrogel®

Pack SizeOrder Code
25 mL24775-082
100 mL24775-025
500 mL24775-017
1 L24775-041
10 L24775-058
20 L24775-066

 

  • Base Matrix
    Agarose
  • Bead Size
    120 µm
  • Operating Pressure
    < 3 barg (44psi)
  • Pack Volume
    500 mL
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Format
    Bulk resin
  • Resin Type
    Mixed Mode
  • Storage Conditions
    20% EtOH/1 M NaCl

 

  • Flow Rate
    < 100 cm/h
  • Ligand
    Hydroxyapatite
  • Reference Protein
    Cytochrome C
  • Typical Dynamic Binding Capacity 10% per mL
    > 7 mg/mL Cytochrome C
  • What you receive
    1 bottle resin, instructions
  • pH Stability
    5 to 12 (working pH)

 

  • Application
    Preparative protein purification
  • Pack Size
    1
  • Quality Standards
    • CoA
    • RSF