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Sartobind STIC PA® 96-Well Plates

Sartobind STIC® PA (primary amine) anion exchange membrane overcomes reduced binding capacity for host cell protein removal and can be used in high-salt conditions.

Item no.:  99STPA42GC-----D


Sartobind STIC® PA removes contaminants at high salt. Salt-tolerant interaction chromatography (STIC) with PA ligand is based on anion-exchange chromatography (AEX) principles. AEX is the established method for removing process-derived contaminants such as host-cell proteins (HCP), DNA, endotoxins, and adventitious and endogenous viruses. It is performed during the downstream processing of monoclonal antibodies (mAbs) in flow-through mode. The process is driven by throughput and not by mass, and thus membrane chromatography (MC) is advantageous over columns for contaminant removal, as membranes run up to two orders of magnitude higher flow rates. Membranes are easy to operate and can be disposed of after one use, just like filters, to save on validation cost.


Current mAb downstream processes consist of at least one initial capture and one AEX chromatography step. The limitation of AEX steps is often that the Q chemistry requires low-salt conditions. In processes with cation exchange (CEX) as the second chromatography step, the CEX pool needs to be diluted to conductivities of 4-7 mS/cm to reestablish binding conditions for the following AEX step. The Sartobind STIC PA membrane is composed of cross-linked, regenerated macro-porous cellulose. The primary amine ligand is attached to the matrix at approximately six-fold higher ligand density than that of Q membrane. Both the free amine and the high ligand density were crucial for developing its high salt tolerance.


The Sartobind STIC® adsorber speeds up the production process by high flow rate and opens new opportunities for the purification of therapeutic proteins and vaccines.

  • No dilution of CEX pools necessary
  • Host cell proteins up to 10kg/l (product load/liter membrane)
  • Viruses >4 log
  • DNA at up to 1.5 M NaCl
  • Endotoxins >4 log

Easy to use:

  • Plug and play devices from 0.08 ml to 1.6 L
  • Can be used like a filter
  • Flow rate up to 30 bed volumes/min
  • Disposable after one use


Sartobind® Membrane Chromatography
en 3.5 MB


Sartobind® 96-Well Plates - High Throughput Screening With IEX and HIC Membrane Chromatography
en 376.4 kB


Sartobind® Selection Guide
en 827.1 kB

User Manual

Directions for Use - Sartobind® 96-well plates — IEX (Q, S, STIC PA) and HIC (Phenyl) in 8-strips — For High Throughput Screening Based on Macroporous Membranes, 0.8 mm Bed Height
en 505.7 kB


  • Application

Connections (Physical)

  • Connectors


  • Bed Height
    0.8 mm
  • Dimensions WxDxH
    128 × 85.5 × 25 mm
  • Membrane Area
    0.7 cm²


  • Reusable

Materials of Construction

  • Housing Material
    Polypropylene (PP)
  • Material 8-strip
    Polypropylene (PP)
  • Material Holding Frame
  • Materials of Construction
  • Membrane Material
    Stabilised reinforced cellulose

Operating Conditions

  • Maximum Operating Pressure
    35 kPa
  • pH Stability
    pH 2 – 14 (short term)

Physicochemical Information

  • Bed Volume
    19 µL/well
  • Ligand
    Primary amine (PA)
  • Ligand Density
    18 – 22 µeq/cm²
  • Pore Size
    3 – 5 µm
  • Reference Protein
    Bovine serum albumin
  • Typical Dynamic Binding Capacity 10% per unit
    0.95 mg/well

Product Information

  • Brand
    Sartobind® STIC PA
  • Membrane Adsorber Format
    96-Well Plates
  • Membrane Type
    Salt tolerant interaction membrane (anion exchanger)
  • Pack Size
  • Type
    Membrane adsorber (96-well plate)
  • What you receive
    • 10 plates
    • 1 operating instruction
Vivawell Vac96 Vacuum Manifold

The Vac96 vacuum manifold has been designed specifically for use with Sartobind® 96 multiwell plates. The long drip nozzles on the outlet eliminate cross-talk between flow channels.