MSCgo™ Chondrogenic Differentiation Medium is a serum-free (SF) and xeno-free (XF) formulation developed for optimal differentiation of human mesenchymal stem cells (hMSC) to mature chondrocytes. The MSCgo™ Chondrogenic Differentiation Medium is validated to efficiently differeniate hMSC from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC), and umbilical cord tissue (UC-MSC).

The MSCgo™ Chondrogenic Differentiation Medium kit includes a basal medium and supplement, containing all growth factors and supplements necessary for a complete chondrogenic differentiation medium.  No additional supplements are required.

The MSCgo™ Chondrogenic Differentiation protocol is part of a complete system for multipotency evaluation of hMSCs.  This kit enables reliable differentiation of hMSCs to mature chondrocytes without background differentiation or interruption in cellular metabolism.

Features

• Serum-free, xeno-free medium
• All required growth factors and supplements included in kit
• Reliable differentiation to mature chondrocytes
• Each lot is application tested
• Does not contain antibiotics

Components

• MSCgo Chondrogenic Differentiation Basal Medium:  100 mL
• MSCgo Chondrogenic Differentiation Supplement Mix :  10 mL

Chondrogenesis Results

Chondrogenic differentiation of hMSC in 3D spheroid culture in the formation of cartilage with a typical extracellular matrix rich of Aggrecan. Aggrecan is a proteoglycan that can be used as an indicator for cartilage formation and can be detected with Alcian Blue, a dark-blue copper-containing dye that acts as an indication of mature chondrocytes. The staining intensity can be vary using different hMSC (e.g. source, age, and passage number).