High-Throughput Surface Plasmon Resonance (SPR)

For Accurate and Rapid Biologic Discovery

The early identification of a compound’s kinetics, affinity and competitive properties is crucial for making accurate decisions in the initial stages of the drug discovery process. This important phase can often be exacerbated by the requirement to investigate large compound libraries to identify molecules with the most advantageous biological properties.

Surface Plasmon Resonance Solutions

The entire Surface Plasmon Resonance (SPR) range by Sartorius has been designed with one clear focus – to allow researchers to generate the most accurate data possible in the shortest timeframe imaginable. 

This is made possible by the use of patented tools exclusive to the Octet® SPR range, which include:

  • OneStep® Gradient Injections: Eliminate the need for multiple analyte concentrations and significantly reduce assay development time to generate kinetics for up to 768 molecules in less than 24 hours
  • OneStep® Two Component Injections: Determine accurate kinetics across multiple conditions and in the presence or absence of cofactors
  • NeXtStep™ Gradient Injections: Highly accurate competition assays to examine the behavior of an analyte in the presence of a competitor molecule

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Octet® SF3 SPR

Octet® SF3 SPR 

Generate high-quality kinetics and affinity data in a fraction of the time compared to standard multi-cycle kinetics using the Octet® SF3.

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Octet CLD Sensor Chip

Octet® SPR Sensor Chips and Consumables

Sensor chip selection is a critical feature of every SPR assay, with each Octet® SPR sensor chip ensuring accurate and reproducible results.

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Octet SF3 SPR system

Octet® SPR Service Contracts

Octet® SPR services detect potential issues before they develop into future problems to help maximize instrument productivity.

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SPR Promotions

Protein Analysis Octet BLI and SPR instruments playing cards
Protein Analysis

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Global - Get up to $190K (€160K) off when you bundle Octet® BLI and SPR systems

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Octet® SPR Solutions Feature:

Octet SF3 SPR Instrument
Octet SF3 SPR Instrument
NeXtStep™ Gradient Injections
Octet SF3 Racks
OneStep® Injection Technology
  • Two standard injection formats: OneStep® Injection Technology and industry standard multi-cycle kinetics (MCK)
  • Eliminate the need to prepare concentration dilution series for each analyte, saving on time, reagents and plate space, and reducing potential errors in assay development
  • Diffuse a single concentration of analyte into a stream of buffer to create an analyte concentration gradient of at least three orders of magnitude 
  • Accurate measurement of binding kinetics and affinity from a single analyte concentration
NeXtStep™ Gradient Injections
  • Determine the behavior of an analyte in the presence of a competitor molecule from a single injection
  • Assess multiple analytes and competitors in a single assay without the need for the competitor to be present in the running buffer 
  • Clearly determine full binding kinetic profiles, affinity and site-specific competition as a modulation of binding in the presence of a competitor molecule
High-Throughput Sample Acquisition
  • Generate complete kinetics and affinity for up to 768 samples in a single unattended assay
  • OneStep® Injection Technology: More than 72 hours unattended run time and a unique sample layout allows for high-throughput acquisition and analysis of hundreds of samples in a single run, making the Octet® SF3 suitable for even the most high-throughput library screens

Surface Plasmon Resonance Resources

Featured Resources

A compendium for successful BLI and SPR assays
Application Guide

A Compendium for Successful BLI and SPR Assays

A comprehensive guide for designing and performing assays that accurately measure the binding kinetics of biomolecular interactions and analyte concen...

Access the Guide
Application Note

Innovative Multi-Cycle Kinetics Replacement in SPR

Multi-cycle kinetics (MCK) is a common analysis performed on SPR systems, where varying concentrations of analyte are tested via separate injections....

Access the Application Note

SPR Downloads

Brochures

Brochure

Octet SF3 Instrument Brochure

PDF | 4.8 MB
Brochure

Octet® SPR Sensor Chips

PDF | 1.3 MB
Brochure

Octet® SF3 SPR Service Plans

PDF | 2.5 MB
Brochure

Octet SF3 Pulse Injection - Brochure | Sartorius

PDF | 1.7 MB

Datasheets and Flyers

Octet SF3 SPR Flyer cover
Flyer

Octet® SF3 SPR Instrument

PDF | 468.7 KB
Flyer

Octet® SF3 Consumables

PDF | 411.3 KB
Flyer

Octet® SF3 SPR Maintenance

PDF | 601.7 KB
The Next Generation of SPR-Based Interaction Analysis cover
Flyer

The Next Generation SPR-Based Interaction Analysis

PDF | 273.6 KB
Flyer

Octet® SPR Sensor Chip Selection Guide

PDF | 591.8 KB

Guides

Best Practice Guide: Octet® SF3 Assay Orientation

PDF | 940.3 KB

Best Practice Guide: Minimize Artifacts 

PDF | 861.4 KB

Best Practice Guide: Octet® SF3 System Maintenance

PDF | 187.3 KB

Best Practice Guide: Minimizing the Effects of Non-Specific Binding

PDF | 120.0 KB

Best Practice Guide: Double Reference Subtraction

PDF | 868.5 KB
Best Practice Guide: Octet SPR Sensor Chip Preparation

Best Practice: Octet® SPR Sensor Chip Preparation

PDF | 99.2 KB
Octet SPR Analysis User Guide
User Guide

Octet® SPR Analysis User Guide

PDF | 2.5 MB
Octet SPR Discovery Software User Guide
User Guide

Octet® SPR Discovery Software User Guide

PDF | 7.6 MB

Application Notes

application note thumbnail
Application Note

Kinetics Determination of High Affinity Molecular Interactions Using OneStep® Injections

PDF | 212.2 KB
Application Note

Kinetic Curvature: Assessment of Small Molecule Kinetics and Affinity Using OneStep® Injections in SPR Screening

PDF | 289.9 KB
SPR Fragment based drug discovery
Application Note

Fragment-Based Drug Discovery (FBDD) Using Octet® SPR OneStep® and NeXtStep™ Injections

PDF | 5.1 MB
OneStep lead characterization of high affinity biologic interactions
Application Note

OneStep® Lead Characterization of High Affinity Biologic Interactions

PDF | 2.2 MB
Application Note

Recovery of Sample Using the Octet® SF3 - Application Note | Sartorius

PDF | 1.4 MB

Technical Notes

Guidelines for OneStep®️ Assay Design

PDF | 3.4 MB

Frequently Asked Questions

Understanding Surface Plasmon Resonance

SPR sensor chips typically consist of a thin metal film, commonly gold, deposited on a glass substrate. The metal film is coated with a thin layer of a biocompatible material, often a self-assembled monolayer, to which the biomolecules of interest can be immobilized.

The sensor surface is then exposed to a liquid sample containing the analytes or target molecules. The interaction between the immobilized ligands and the analytes leads to changes in the refractive index at the surface, which can be detected and analyzed in real time.

SPR-based biosensors rely on the detection of changes in the evanescent electromagnetic field that occurs at the metal-dielectric interface when the surface plasmons are excited.

The excitation of surface plasmons is achieved by coupling a beam of polarized light, to the metal film at a specific angle known as the resonance angle. This angle is determined by the refractive index of the sample medium and the properties of the metal film.

The resonance condition is highly sensitive to changes at the metal-dielectric interface, such as the binding of a biomolecule, making SPR a powerful tool for studying biomolecular interactions.

When the incident light beam hits the metal-dielectric interface at the resonance angle, it generates an evanescent wave that extends into the sample medium. This evanescent wave interacts with the analytes in the sample, leading to changes in the refractive index near the surface. These changes can be detected as alterations in the intensity or angle of the reflected light.

A detector measures these changes, and the resulting signal is processed to obtain information about the binding events occurring on the sensor surface. By monitoring the changes in the SPR signal over time, scientists can gain valuable insights into the kinetics and affinity of biomolecular interactions of the binding process.

The rate at which the signal changes provides information about the association and dissociation of the analytes with the immobilized ligands, allowing the calculation of kinetic parameters such as association rate constants (ka or kon) and dissociation rate constants (kd or koff). The equilibrium binding affinity (KD) can also be calculated as the ratio of kd and ka or also be determined by analyzing the steady-state signal.

SPR-based biosensors enable the monitoring of dynamic processes and the analysis of fast-binding interactions. They are also highly sensitive, allowing the detection of small molecule analytes at low concentrations.

SPR technology has a wide range of applications, including drug discovery, antibody screening, protein-protein interactions, and nucleic acid interactions which provide valuable information about binding kinetics, analyte concentration, and the thermodynamics of these interactions in a label-free and real-time manner, making it an indispensable tool in many research and development settings.

Surface Plasmon Resonance is a cutting-edge technology that provides a powerful tool for studying and understanding molecular interactions. It's a key player in advancing our knowledge in biochemistry and related fields, contributing significantly to the development of new drugs and therapies.

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