Specialized Solutions for Concentration and Buffer Exchange

Vivaspin® Pressure Cells, Vivapore® Static Ultrafilters and Ultrafiltration Membrane Discs

While centrifugal filters have become the gold standard for rapid concentration and buffer exchange of small sample volumes, they are not always the most practical choice.

For special and customized applications, such as single or high throughput sample processing, Sartorius is proud to offer ultrafilters with unique operating methods that enable low-shear processing with reduced or no equipment requirements. These include ultrafilters driven by positive pressure or solvent absorption, accessories for low-volume continuous diafiltration, and ultrafiltration membrane discs.

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Vivaspin® 20 and 100

The world’s only pressure-ready centrifugal filters for low-shear UF | DF of single or sensitive 5-100 mL samples

Air Pressure Controller

Vivaspin® Pressure Accessories

Built for simple pressurization of Vivaspin® 20 | 100 ultrafilters with intuitive control and over-pressure protection

Vivapore®

Solvent absorption ultrafilters for high throughput, equipment-free benchtop concentration

Unisart membranes

Ultrafiltration Membranes

Membrane discs in PES, RC and CTA and multiple diameters for legacy stirred cells and other applications

Pressure Cell and Static Ultrafilters Offer:

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Compact Footprint

Small by design. Pressure cell and solvent absorption formats take up minimal space for clutter-free benchtop or refrigerated sample concentration.

Gentle Processing

Take the pressure off your sample. For sensitive targets such as viruses and multiprotein complexes, low-shear techniques can improve recoveries and prevent degradation.

One-Off Simplicity

There is no need to balance a rotor when you’re not using a centrifuge. Handle single samples easily, equipment-free or with a readily available compressed gas source.

Increased Intensity

High throughput, equipment-free. In research labs or the clinic, handling hundreds of similar samples daily shouldn’t increase demand on your centrifuge or risk carryover.

Effortless Collection

Pipette-friendly to the last drop. Avoid target loss with sample-safe dead stops that are perfectly complemented by Optifit tips for easy retentate collection.

Easy Monitoring

Track in a snap. A quick glance at the printed graduations or concentration factors will tell you if your process is complete or if you need to run it a little longer.

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Pressure Cell and Static Ultrafilters and Their Applications

Positive Pressure Ultrafiltration

Compressed air or an inert gas is used to create a pressure difference across the ultrafiltration membranes that drives the separation of particles or macromolecules from liquids based on their size or molecular weight. A sample (feed) containing the molecule of interest is dispensed into a filter unit equipped with ultrafiltration membranes. When gas pressure is applied, larger molecules in the sample are retained above the membrane (retentate), while smaller molecules and solvent pass through (permeate).

Need a low-shear solution for processing larger sample volumes?

Explore Lab TFF Cassettes

Positive pressure ultrafiltration is a convenient centrifuge-free alternative for UF | DF of small to medium sample volumes.


Faster Processing Under Pressure

For even faster ultrafiltration and diafiltration, the pressure difference across the membrane can be applied through two sources. In Vivaspin®️ 20, positive pressure can be combined with centrifugation (pressure-fugation), while Vivaspin®️ 100 can be continuously pressurized and the ultrafilter placed on an orbital shaker (pressure-shake).

Ready to try Vivaspin® as a pressure cell ultrafilter?

Buy Pressure Accessories

Pressure-fugation and pressure-shake operation can be used to increase the speed of positive pressure UF | DF.


Advantages of Pressure Cells

Positive pressure ultrafiltration is a low-shear alternative to centrifugal filters and legacy stirred cells, making it especially valuable when handling sensitive samples such as membrane proteins, enveloped viruses, and multi-unit protein complexes. It is also a useful method when only a single sample must be concentrated, or when a refrigerated centrifuge is unavailable for processing temperature-sensitive samples. A further advantage of pressure cells compared to stirred cells is that there is no risk of concentrating samples to dryness.

Need a reliable way to concentrate membrane proteins?

Read our Application Note

Positive pressure ultrafiltration is an ideal solution to process shear-sensitive samples without the risk of degradation.


Keep Still and Concentrate

When access to a centrifuge is limited and a large number of samples must be processed on a daily basis, centrifugal ultrafiltration is impractical. Vivapore® ultrafilters passively concentrate samples by solvent absorption, which doesn’t require any equipment. These single units have a compact footprint for bench-top concentration without the risk of cross-contamination. Ultrafiltration process monitoring is possible at-a-glance with clear volume and concentration factor markings, while sample loss to dryness is eliminated.

Interested in making your buffer exchange process more efficient?

Learn More About Diafiltration

Choose Vivapore® for your research or clinical lab to concentrate proteins >15 kDa, equipment-free.

Lab Filtration & Purification Resources

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Selected Centrifugal Filter Resources

Vivaspin® - Comprehensive Concentration Solutions
Datasheet

Vivaspin®

Learn more about the only centrifugal ultrafilters that can be used without a centrifuge.

Air Pressure Controller - For Pressurized Ultrafiltration of Sensitive Targets
Datasheet

Pressure Cell Accessories

Find the specifications and ordering information for the APC, Vivaspin® pressure heads, and fittings.

product datasheet
Datasheet

Vivapore®

Refer to the technical specifications and ordering information for solvent absorption ultrafilters.

Specialist Ultrafilter Manuals

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Frequently Asked Questions

Sartorius offers special ultrafilters with the following MWCO | pore size membranes:

For more detailed information on membrane availability, please refer to the product datasheets.

12 or 48 units (Vivaspin® 20), or 2 or 10 units (Vivaspin® 100), with standard caps (for centrifugal operation), and a quick start guide.

30 ultrafilters, one stand for four units, and a quick start guide.
Each pack of Vivapore® 10 also includes one expansion reservoir.

10 ultrafiltration membranes.

Yes, they are. While we have taken steps to minimize our environmental impact with quick start guides in the product packaging, if you need more information on how to use our products, the full instruction manual can be downloaded from this page or in the eShop.

For either of these ultrafilters, a pressure regulator such as the Air Pressure Controller must be connected to a compressed gas source (e.g., air, argon or nitrogen cylinder).

In addition, Vivaspin® 20 requires a pressure head and a charge valve, while Vivaspin® 100 requires a pressure head and seal.

Yes. While each pressure head is already supplied with five seals, replacement seals can be ordered separately when required.

Yes, you can. Using Vivaspin® 20, large sample volumes should either be concentrated to 2 mL or less, or divided between several ultrafilters in 2 mL aliquots. Insert a diafiltration cup into each ultrafilter, above the sample, and fill with 10 mL exchange buffer. When centrifuged, diafiltration occurs at the same rate as ultrafiltration.

Each pack of Vivapore® ultrafilters already includes one stand to hold four units. Additional stands are available to purchase separately. Sartorius recommends using glass Pasteur pipettes to dispense samples into the ultrafilters and collect the retentates.

The performance of ultrafiltration membranes degrades with repeated use, so Vivaspin® 20 and Vivapore® centrifugal filters are designed for single use. This helps to reduce hands-on time, improve laboratory safety, and eliminate the risk of carryover.

However, cleaning and storage procedures are provided for Vivaspin® 100 so that they can be used multiple times.

In general, Hydrosart® RC membranes are easier to clean than other membrane materials, but you should consider if the risk of carryover and the additional time and materials required for cleaning outweigh the benefits of single use for each application.

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