Octet® Software Tutorials
Mastering Octet® BLI Discovery and Analysis Studio Software for Streamlined Biomolecular Interaction Analysis
Octet® BLI Discovery and Analysis Studio Software are powerful tools designed to simplify data acquisition and analysis for label-free biomolecular interaction studies using the genuine BLI platform. These software suites offer an intuitive interface, pre-defined templates, and advanced analysis capabilities, enabling researchers to efficiently characterize a wide range of biomolecular interactions.
In this collection of tutorial videos, we will explore the key features and functionalities of both software packages, providing you with the knowledge to master these tools and streamline your biomolecular interaction analysis workflow.
Octet® BLI Discovery Software: Seamless Data Acquisition
Octet® Tutorial 1
New to the Octet®: Where to start?
Operating a new software system can be overwhelming. To help those who require a little guidance, we’ve designed a series of easily digestible videos explaining everything you need to get going with your Octet® software. In this tutorial, you gain an overview of the three types of Octet® software, their main functionalities and the key differences.
Octet® Tutorial 2
New to the Octet®: Brief introduction to data acquisition software
This video tutorial takes you through the basics of the Octet® Data Acquisition Software. This software is all about running assays and it is the main application from which you will set your control parameters. Learn everything from loading method files and setting sample plate temperature to editing assay parameters and changing sensor types.
Octet® Tutorial 3
New to the Octet®: Brief introduction to data analysis HT software
Tutorial 3 takes a look into our Octet® Data Analysis HT software. This software is designed for those requiring high throughput analysis. Learn the basics of the software such as opening your dataset, the overlay feature, and how you can concatenate different experiments together. Find also some useful tips and tricks to make your analysis easier!
Octet® Tutorial 4
Direct quantitation with regeneration - Part 1
In this video tutorial, you take a look into assay design, how to setup a direct quantitation of an analyte, and the regeneration of the biosensor surface. Learn in part 1 how to setup the plate definition of your experiment.
Octet® Tutorial 5
Direct quantitation with regeneration - Part 2
In this video tutorial, you take another look into assay design, how to setup a direct quantitation of an analyte and the regeneration of the biosensor surface. Learn in part 2 how to manage the sensor assignment, review and run your biosensor experiment.
Octet® Tutorial 6
How to set-up a kinetic assay?
The tutorial explains the assay wizard in Data Acquisition HT Software version 12.
Octet® Analysis Studio Software: Powerful Data Analysis
Octet® Tutorial 7
How to analyze a quantitation assay: Preprocessing data?
The tutorial presents how to analyze data of a quantitation assay. Learn in part 1 how to preprocess your data for the best results of your biosensor application.
Octet® Tutorial 8
How to analyze a quantitation assay: Quantitation analysis?
This video tutorial takes a look into analyzing data of a quantitation assay. Learn in part 2 how to carry out the analysis of your data set for the best results of your biosensor application.
Octet® Tutorial 9
How to assess the quality of analyzed quantitation data?
This video tutorial looks into the quality of analyzed quantitation data. There are three steps to assess the quality: the quality of the fit of raw data (R2), the quality of the resulting standard curve and the similarity between replicates.
Octet® Tutorial 10
What is a kinetic assay?
Video tutorial 10 describes kinetic assays on Octet® instruments for biomolecular interaction analysis. Fundamentals of association, steady-state and dissociation were explained.
Octet® Tutorial 11
What is a loading scout?
This video tutorial gives a general description of a loading scout. It leads through a loading scout - how it looks and why it is so important to start a new kinetic assay development with the loading scout.
Octet® Tutorial 12
Which concentrations should be used in a loading scout?
Tutorial 12 explains the importance of concentrations to be used, both the one for the ligand and one for the analyte.
Octet® Tutorial 13
How to setup a loading scout method file?
In this video tutorial, the setup of a method file in the data acquisition software on the Octet® instrument is presented.
Octet® Tutorial 14
How to analyze a loading scout?
This video tutorial shows how to analyze a loading scout experiment in the Data Analysis HT Software version 12.
Octet® Tutorial 15
How to analyze a kinetic assay? Preprocessing data - sensor & sample subtraction
In this video tutorial, you will learn to analyze kinetic data measured on the Octet® platform. In this tutorial, the focus is on best practices for the data preparation to carry out the kinetic analysis.
Octet® Tutorial 16
How to analyze a kinetic assay? Preprocessing data - Data correction
In this video tutorial, you will learn further options to analyze kinetic data measured on the Octet® platform.
Octet® Tutorial 17
Kinetic analysis
In this tutorial, the focus is on the kinetic analysis itself. Learn about a full global fit with a 1:1 binding model including association and dissociation.
Octet® Tutorial 18
Quality of the fit
In this video tutorial, the focus is on the quality of the fitted data.
Quick Start Guides
Questions or Need Help?
If you have any questions or need further assistance while exploring our Octet® BLI Discovery and Analysis Studio Software, please don't hesitate to reach out. Our team is here to support you in mastering the software's features, from kinetics and affinity data acquisition to advanced biomolecular interaction analysis and quantification applications. We're committed to helping you achieve your research and analysis goals with confidence.
