Sartorius PSC Optimization and Characterization Solution
Scaled-up, semi-automated, high-throughput expansion and characterization solution to develop process insight through CPP and CQA correlation for a robust iPSC manufacturing protocol
- Process control: Determine optimal expansion and differentiation process in controlled cell cultures (pH, DO, temperature, stirring speed) of iPSCs and derivatives with consistent, serum-free, xeno-free iPSC expansion medium and 3-hour sterility checkpoints in 100-250mL cultures
- Automation: Minimize manual time with a multi-parallel platform and walkaway automation advanced flow cytometry and continuous live-cell analysis platforms
- At-scale throughput: Optimize in 1-8 bioreactors operated in parallel and characterize cells in suspension and adherent cells in 96- and 384-well plates
- Extensive characterization: Acquire differentiation marker information using advanced flow cytometry and detect morphological changes during differentiation using continuous live-cell analysis
- Process insight: Gain rapid understanding of the optimal expansion and differentiation protocol by analyzing process parameters and characterization data to correlate CPPs and CQAs
Process Control
Ambr® 250 Modular
Expandable automated benchtop bioreactor system with 2-8 single-use 100-250 mL vessels for accelerated, high-throughput PSC process optimization
MODDE®
Design of experiment software to enable a seamless transition from planar to suspension culture while identifying critical process parameters
NutriStem® hPSC XF
Defined, serum-free, xeno-free PSC expansion medium to support expansion of undifferentiated hPSCs. FDA DMF on file
NutriFreez® D10 Cryopreservation Media
Fully defined, serum-free cryopreservation medium for a consistent, high-performance protective environment for PSCs during the freezing, storage, and thawing
Microsart® ATMP
3-hr PCR-based kit for the detection of mycoplasma, bacteria, and fungi for in-process testing of PSC therapeutics
Automation
Ambr® 250 Modular
Expandable automated benchtop bioreactor system with 2-8 single-use 100-250 mL vessels for accelerated, high-throughput PSC process optimization
iQue® 3
Advanced high-throughput flow cytometry platform for phenotypic analysis of PSC and PSC derivatives and differentiation tracking
Incucyte® SX5
Automated, real-time, live-cell imaging and analysis platform for quantitative insights into PSC and PSC derivative health, morphology, proliferation and function
MODDE®
Design of experiment software to enable a seamless transition from planar to suspension culture while identifying critical process parameters
At-Scale Throughput
iQue® 3
Advanced high-throughput flow cytometry platform for phenotypic analysis of PSC and PSC derivatives and differentiation tracking
Incucyte® SX5
Automated, real-time, live-cell imaging and analysis platform for quantitative insights into PSC and PSC derivative health, morphology, proliferation and function
Ambr® 250 Modular
Expandable automated benchtop bioreactor system with 2-8 single-use 100-250 mL vessels for accelerated, high-throughput PSC process optimization
MODDE®
Design of experiment software to enable a seamless transition from planar to suspension culture while identifying critical process parameters
Extensive Characterization
Incucyte® SX5
Automated, real-time, live-cell imaging and analysis platform for quantitative insights into PSC and PSC derivative health, morphology, proliferation and function
iQue® 3
Advanced high-throughput flow cytometry platform for phenotypic analysis of PSC and PSC derivatives and differentiation tracking
Process Insight
SIMCA®
Powerful multivariate data analysis tool with interactive visualizations, an intuitive interface, and automatable workflows for comprehensive process understanding
