Sartorius T-Cell Screening Solution
A semi-automated solution for multiplexed phenotyping and functional characterization of engineered T-cells for identification of critical quality attributes, CAR construct optimization, and validation of CAR construct screens to enable discovery of markers expressed on target cancer cells and of more potent CAR constructs for established cancer markers with fewer off-target effects.
- Control variability: Ensure reproducibility of your observations by using consistent serum-free medium, precise pipetting tools, and consistent water as well as by implementing mycoplasma, bacteria, and fungal checkpoints
- Automation: Perform functional and phenotypic characterization of CAR-T cells to discover high-affinity CAR constructs for specific targets on cancer cells and validate your findings in a flexible assay platform with DoE software package
- At-scale throughput: Acquire cytokine, phenotype, and function information at throughput with 24-48 sample flexible assay platform, multiplex analysis of cells in suspension, and continuous live-cell analysis in 96- and 384-well plates
- Extensive characterization: Characterize CAR affinity, potency, and off-target effects through information-rich T-cell phenotyping, cytokine analysis, and T-cell killing assays
- Insight: Obtain a comprehensive understanding of the CAR receptor and of the CAR-T cell phenotype and in vitro and in vivo function
Control Variability
4Cell® Nutri-T
Xeno-free, serum-free medium specifically designed for the culture and expansion of lymphocytes
Microsart® Research
3-hr PCR-based kit for the detection of mycoplasma, bacteria, and fungi for contamination control
Ambr® 15
High throughput, automated bioreactor system for 24 or 48 parallel cultivations at the 10 –15 mL microbioreactor scale
MODDE®
Design of experiment software to facilitate translation of data to insights and shift process complexity from the floor to the control room
Picus® Nxt
Ergonomic electronic single- and multichannel pipettes with protocol memory and with adherence to the strictest regulations
Sartoclear Dynamics® Lab
Out-of-the-box, fast and effortless kit to clarify high-density mammalian cell cultures
Vivaspin®
Ultrafiltration device with unique twin vertical membranes for optimal crossflow, 0.5 to 15 mL
Vivaflow®
Scalable crossflow ultrafiltration concentrator, 100 mL to 5 L
Centrisart® G-16C
High-capacity, refrigerated bench top centrifuge for harvesting T-cells directly in Ambr® 15 buckets
Automation
iQue® 3
High-throughput, advanced flow cytometry platform for T-cell activation and cytokine profiling assays as well as multiplexed phenotypic analysis
Octet® HTX
Label-free, ultra high-throughput system for receptor-antigen affinity and kinetics measurement
Incucyte® SX5
Automated, real-time, quantitative, live-cell imaging and analysis platform for T-cell homing (chemotaxis), activation and function assays
Ambr® 15
High throughput, automated bioreactor system for 24 or 48 parallel cultivations at the 10 –15 mL microbioreactor scale
MODDE®
Design of experiment software to facilitate translation of data to insights and shift process complexity from the floor to the control room
At-Scale Throughput
iQue® 3
High-throughput, advanced flow cytometry platform for T-cell activation and cytokine profiling assays as well as multiplexed phenotypic analysis
Incucyte® SX5
Automated, real-time, quantitative, live-cell imaging and analysis platform for T-cell homing (chemotaxis), activation and function assays
Octet® HTX
Label-free, ultra high-throughput system for receptor-antigen affinity and kinetics measurement
Ambr® 15
High throughput, automated bioreactor system for 24 or 48 parallel cultivations at the 10 –15 mL microbioreactor scale
MODDE®
Design of experiment software to facilitate translation of data to insights and shift process complexity from the floor to the control room
Extensive Characterization
iQue® 3
High-throughput, advanced flow cytometry platform for T-cell activation and cytokine profiling assays as well as multiplexed phenotypic analysis
Incucyte® SX5
Automated, real-time, quantitative, live-cell imaging and analysis platform for T-cell homing (chemotaxis), activation and function assays
Octet® HTX
Label-free, ultra high-throughput system for receptor-antigen affinity and kinetics measurement
Insight
iQue Forecyt® Software
Interactive assay development, analysis, and multiparameter data visualization
Incucyte® Software
Powerful phenotypic cellular analysis, including quantification of dynamic changes in cell subsets and chemotaxis
Octet® Systems Software
Data acquisition and analysis, including kinetics, quantitation, and epitope binning assays
