The Octet^® SPR HisCap sensor chip consists of preimmobilized Nitrilotriacetic acid (NTA) within a nondextran polysaccharide 3D surface, which can be used for affinity capture coupling. In comparison to the strong interaction between streptavidin and biotin, the lower bond strength observed between NTA and a His-tag allows the easy removal of His-tagged ligand proteins and the regeneration of sensor chip surfaces using a variety of conditions, including imidazole, SDS and EDTA.