HEA HyperCel RoboColumn - 200 µL

Tech Support Documents
HEA HyperCel RoboColumn - 200 µL
 

HEA HyperCel is a mixed-mode resin with a less hydrophobic hexylamine ligand allowing protein binding at a moderate salt concentration under neutral pH. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

Price (USD):
$341.00
Item No.: 
SR2HEA

*Custom/bulk order quotes are provided within 72 hours of request.

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HEA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a hexylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. HEA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).

Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


Applications

- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC

Applications

  • Application
    HTPD resin screening  

Compliance Information

  • Quality Standards
    CoC  

Dimensions

  • Bead Size
    80 - 100 µm  
  • Bed Height
    10 mm  
  • Dimensions
    5 x 10 mm  

General Specifications

  • Flow Rate
    16-1000 cm/hr  
  • Resin Format
    Pre-packed column  

Materials of Construction

  • Base Matrix
    Rigid cellulose beads  
  • Materials of Construction
    Polypropylene  

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)  
  • pH Stability
    1 to 14 (short term), 2 to 12 (long term)  

Physicochemical Information

  • Bed Volume
    200 µL per column  
  • Ligand
    Hexylamine  
  • Reference Protein
    Bovine serum albumin (BSA)  
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA  

Product Information

  • Brand
    HEA HyperCel  
  • Pack Size
    1 row of 8  
  • Pack Volume
    200 µL  
  • Resin Delivery Condition
    1M NaCl with 20% EtOH  
  • Resin Type
    Mixed-mode anion exchanger  
  • What you receive
    1 row of 8 columns  

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl