Enables Users to Identify Hits Faster, Obtain More Information-Rich Data, and Eliminate Sample Dilutions

  • Simplified kinetic characterizations - With OneStep injections, users can obtain accurate kinetic and affinity values from one analyte concentration eliminating the need to run multiple analyte titrations saving time and error associated with making dilutions. NextStep injections provide a simple workflow to characterize competitive binders against a ligand to capture the mechanism of action.
  • Robust fluidics design - Pioneer fluidic system is made up of inert materials such as PEEK, ceramic and Tefzal providing durability and low maintenance.
  • Low baseline noise, minimal drift, high sensitivity and up to 40 Hz data collection rate - Extremely low baseline noise and drift enable measurement of reliable kinetics and affinity values for high affinity and challenging binders. With up to 40 Hz of data acquisition capabilities, Pioneer FE systems are capable of capturing very fast kinetic association and dissociation phases of a wide range of biomolecules and small molecule fragments.

Pioneer SPR systems provide high quality kinetic data (ka, kd) and affinity measurements (KD) to chracterize a wide range of biomolecular interactions from small molecule fragments to biologics. OneStep SPR injections enable faster assay workflows where a single analyte concetration is sufficient for accurate kinetics and affinity determination.

Pioneer SPR System
Three-channel, fully automated SPR system

With three sensing channels, two target proteins or ligands can be analyzed simultaneously for binding with one surface utilized as a reference channel.

Up to 72 hours of unattended run time

Offers a variety of sample vessel formats with up to two 384 well plate capacity with an unattended runtime of about 72 hours for performing large binding screens.

Rapid data analysis with QDat software

Pioneer SPR systems are equipped with an integrated, industry-proven data analysis software based on Scrubber and Clamp platforms. Raw data across multiple runs, days, and sensors can be combined and analyzed using binding algorithms such as single site, multi-site binding, mass transport, and irreversible inhibitor analysis as required for analysis.

Gain more data with less

As the analyte gradient is generated in OneStep injections, the diffusion coefficient is calculated that provides an assessment of aggregation enabling users to gain information not only on kinetics and affinity but also on aggregation in just one injection.

Robust fluidics design

The Pioneer fluidics system is made up of inert materials such as PEEK, ceramic and Tefzal providing durability and low maintenance.

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Applications

Characterization of High Affinity Biologic Interactions

Target binding characterization is an essential analytical step for the selection of high affinity (KD <1 nM) and highly specific biologics regardless of the types of molecules. A kinetic analysis further describes the components of association and dissociation that comprise the overall affinity interaction. For example, two lead compounds may possess a similar affinity (KD) to its target, but their differences in kinetic rate constants of association and dissociation can be used to estimate which will be more useful in vivo. Accurate analysis of these kinetic rate constants is therefore important information for lead selection and predicting the efficacy of protein therapeutics.

The Pioneer SPR system with next generation SPR injections improves the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. The next-generation OneStep gradient injection featured on the Pioneer platform dramatically increases the speed of affinity characterization while maintaining accuracy and high confidence in results.

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Characterize Irreversible Inhibitors and Measure Commitment to Covalency

The majority of small molecule inhibitor assays tested with label-free, real-time biosensor technologies are reversible interactions, characterized by commonly used kinetic rate models. However, a significant fraction of therapeutic enzyme inhibitors on the market functions through covalent modification of the target.

The Pioneer is a SPR platform that can be used with regenerable Streptavidin biosensors to reversibly capture protein targets and quantify the efficiency of covalent inhibitors binding to the target. The Pioneer system's irreversible inhibitor applications method can be used to determine inhibitor compound's commitment to covalency (Cc) as a metric for irreversible inhibitors.

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Featured Publications

Label-Free Detection Technologies: Key Considerations and Applications

Binding interactions in biological samples are dynamic and driven by changes to the environment. The techniques used to characterize these...

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Refractive Index Range

1.33–1.40

Short Term Noise

< 0.1 RU

Molecular Weight Cutoff

< 70 Da

Working Rangeska 102 – 108 M-1 s-1
kd 10-6 – 0.1 s-1
Sample Capacity

2 sample racks

Sample Configuration

96 vial, deep well and PCR formats, 384-well microplates, custom high volume

Sample Temperature Control

4 to 40°C (max 15° below ambient)

Sample loading

Automatic

Number of Channels

3

Flow Channel Volume

< 90nL

Reference Curve Subtraction

Yes

Injection Volume

2–700 μL

Simultaneous Injections

Yes, dual sample loops

Inline Buffer Degassing

Yes

System Temperature Control

4–40°C (Max 15° below ambient)

Variable Data Rate

1–20 Hz

Automation Capabilities

> 72 hour unattended operation

Qdat hit selection feature

No

High-throughput screening mode

No

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