Sialic Acid (GlyS) Kit

High Throughput Glycan Screening of Crude and Purified Samples

  • No sample purification or digestion required; reduces preparation time by up to 3 hours
  • Screen for samples with desired sialylation levels – high, medium or low
  • Combine titer data with sialic acid data for more informed decisions

Glycosylation is considered among the most important post-translational modifications when developing new biologics.

Having a significant impact on product performance and variability, glycosylation is a critical quality attribute (CQA) influencing product safety and efficacy.

Protein glycosylation can affect isolation and purification steps (process consistency), pharmacokinetics (half-life) properties and in vitro stability (product shelf-life). Sialic acid content is especially important as it can impact the stability and clearance of a protein.

The Sialic Acid (GlyS) Kit provides a rapid and convenient method for relative screening of terminal sialic acid content in crude or purified samples. Sialic acid data can be combined with titer data using Octet® Data Analysis HT software version 11.1 or higher to funnel through samples that are high producers and have desirable sialic acid content.

Human IgG or Human Fc-fusion Proteins Workflow

Selective amplification of signal is from the protein of interest and not from host-cell protein (HCP).

Comparison of GlyS Throughput on Various Octet® BLI Systems

Assay ParameterOctet® R8 System, Octet® AS Instrument and GlyS KitOctet® RH16 System, Octet® AS Instrument and GlyS KitOctet® RH96 System and GlyS Kit

Time to Results

<2.5 hr/96 samples

˜1.5 hr/96 samples

<1 hr/96 samples

Sample Type

Crude cell culture supernatant

Crude cell culture supernatant

Crude cell culture supernatant

Sialic Acid (GlyS) Kit

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Related Resources

Octet® Sialic Acid (GlyS) Kit

Octet® Sialic Acid (GlyS) Kit

PDF | 489.3 KB
cell live development app note cover
Application Note

Cell Line Development: Antibody Discovery by Monitoring Titer and Glycosylation with the Octet® Platform

PDF | 300.1 KB
selection guide

Octet® BLI Biosensor Selection Guide

PDF | 404.8 KB

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