Sialic Acid (GlyS) Kit

High Throughput Glycan Screening of Crude and Purified Samples

• No sample purification or digestion required; reduces preparation time by up to 3 hours
• Screen for samples with desired sialylation levels – high, medium or low
• Combine titer data with sialic acid data for more informed decisions

Glycosylation is considered among the most important post-translational modifications when developing new biologics.

Having a significant impact on product performance and variability, glycosylation is a critical quality attribute (CQA) influencing product safety and efficacy.

Protein glycosylation can affect isolation and purification steps (process consistency), pharmacokinetics (half-life) properties and in vitro stability (product shelf-life). Sialic acid content is especially important as it can impact the stability and clearance of a protein.

The Sialic Acid (GlyS) Kit provides a rapid and convenient method for relative screening of terminal sialic acid content in crude or purified samples. Sialic acid data can be combined with titer data using Octet^® Data Analysis HT software version 11.1 or higher to funnel through samples that are high producers and have desirable sialic acid content.