Streamline Commercial TFF with a Flexible, Reliable, Efficient Solution
Maximize TFF Performance With an Automated and Scalable System
Sartoflow® 5000 SU is designed for fully automated ultrafiltration, diafiltration, and microfiltration processes, and engineered for commercial-scale bioprocessing from 200 to 2,000 L and up to 15 kg of product. The compact system delivers superior performance and reliability with low minimum working volumes and filtration areas up to 14 m² for cassettes and 5 m² for hollow fibers.
Engineered for high-efficiency, single-use TFF at scale—with automation, speed, and process-ready design.
Mitigate risk with an aseptic and closed flow kit, multi-block valve, built-in visual installation guidance, and consistency through Biobrain® automation.
Seamlessly switch between cassettes and hollow fiber modules through a standardized and open design.
Achieve up to 5,000 L/hr flow rates, with 40% more filter-area capacity than Sartoflow® 4500 and up to 99.9% product recovery.
Explore Sartoflow® 5000 SU
Modular TFF System with Configurable Setup for Cassettes and Hollow Fibers
Sartoflow® 5000 SU is a smart, automated TFF system that integrates hardware, control software, and single-use flow kits for fast setup and flexible operation. Fully automated processes are enabled by a single-use flow path with BioPAT® sensors, actuators, Flexsafe® Pro Mixer technologies, and Biobrain® software suite for reliable and scalable performance.
Biobrain® is a modular automation platform designed to meet the evolving needs of upstream and downstream bioprocessing. It combines industrial-grade hardware and software with full traceability and flexible configuration for rapid adaptation. With OPC UA support for monitoring and control, Biobrain® integrates smoothly with systems like DCS (e.g., DeltaV) and MES, ensuring reliable interoperability. Built to meet industry standards and Bioprocessing 4.0 requirements, it enables faster deployment, easy reconfiguration, and a future-ready digital foundation.
Recirculation Tank
The versatile Flexsafe® tank is designed to hold a mixing bag and connect with a drive unit, a purpose-built for consistent mixing of biopharmaceutical products. Available in 50 L, 200 L, and 400 L sizes (further sizes on request), this tank seamlessly integrates with the Sartoflow® 5000 SU system.
For enhanced functionality, an optional temperature control unit and double-walled tank design are available to enable precise liquid temperature control.
Sartoflow® Cassette Holder Trolleys
The Sartoflow® 5000 SU Solution offers flexible holder trolley options for TFF filter modules, allowing you to choose between an automatic and manual holder for flat sheet cassettes.
The holder trolleys are practical and flexible solutions designed for seamless filtration, accommodating multi-use, single-use cassettes, and self-contained units, supporting up to 14 m² of filtration area (21 m² holder option available on request).
With its advanced features, including automatic clamping force and monitoring, the cassette filter holder ensures efficient and straightforward operation, making it a valuable addition to your filtration process.
Sartoflow® Hollow Fiber Holder Trolley
The hollow fiber holder trolley for the Sartoflow® 5000 SU is specifically designed to securely mount Sartorius hollow fiber modules in different sizes, making it ideal for use in GMP production suites.
Sartoflow® Single-Use Flow Kit
The Sartoflow® Single-Use Flow Kit is carefully designed to integrate effortlessly with the Sartoflow® 5000 SU system, ensuring optimal performance and compliance with industry standards. Built around core components manufactured by Sartorius and carefully selected third-party components, the flow kit adheres to common guidelines, guaranteeing suitability and reliability.
Sartoflow® Single-Use Bags
The Flexsafe® Pro Mixer Bag, specifically designed for the Sartoflow® 5000 SU, combines ergonomic and innovative features for exceptional ease of handling.
Its unique side K-weld design simplifies installation and facilitates smooth unfolding and folding during filling and draining operations. Optimized tubing and sensor positioning ensure easy installation and direct access for filling, sampling, and draining. The levitating impeller, with high torque, efficiently mixes high-concentration or viscous solutions while minimizing shear effects.
Available in 50, 200, and 400 L sizes, with Flexsafe® single-use 3D mixing bags supporting efficient mixing and temperature control up to 400 L. An integrated sampling port and sensors for conductivity, pH (> 200L scale), and temperature enable continuous process monitoring.
Customers can choose between three Sartoflow® 5000 SU service level agreements: Essential, Advanced and Comprehensive. Preventive maintenance is an integral part of all contract types.
| Essential | Advanced | Comprehensive | |
|---|---|---|---|
| Plannable annual maintenance | All costs are covered including wear parts | ||
| Reaction time help desk | Within one business day | Within 8 hours | Within 4 hours |
| Reaction time on-site support | Priority | Within 72 hours | Within 48 hours |
| Repair | Discount on all time-and material-based cost elements | All labor and travel costs are covered, a discount of 10% on spare parts (parts charged separately) | All costs are covered |
Learn More About Sartoflow® 5000 SU
Frequently Asked Questions
Achieve high efficiency and flexibility for large-scale bioprocessing with a fully automated, single-use tangential flow filtration (TFF) solution. Benefit from up to 99% product recovery, a 10% improvement in flow rate over previous models, and rapid setup in under 25 minutes. The closed, aseptic design minimizes contamination risk and reduces cleaning and validation costs, supporting consistent, high-quality output for mAbs, ADCs, viral-based therapies, and nucleic acids.
Processing is streamlined with automated controls, optimized flow profiles, and multi-purpose capability for both cassettes and hollow fiber modules. This enables efficient ultrafiltration, diafiltration, and microfiltration across a range of modalities. Batch sizes from 200 to 2,000 liters are supported, operator intervention is reduced, and high product recovery is ensured—making it ideal for high-throughput, multi-product facilities.
Designed for scalability and compliance, this platform supports GMP-ready documentation, global harmonization, and robust automation. The modular design allows for easy configuration, rapid tech transfer, and integration with existing utilities. The closed single-use flow path reduces cross-contamination risk, while advanced software and service support ensure reliable, reproducible performance across global sites.
This solution replaces the Sartoflow® 4500, offering significant upgrades in automation, flow rate (up to 5,000 L/hr), and product recovery. It features a dual-use design for both cassettes and hollow fibers, improved ease of use, and a more compact, user-friendly flow kit. The system is engineered for higher flexibility, faster setup, and lower operational costs, setting a new standard for large-scale TFF applications.
Seamless integration is supported with connectivity to tanks, mixers, and system control units, and compatibility with a wide range of Sartorius consumables and sensors. Automation-ready software, standardized hardware, and configurable options ensure smooth adoption into both single-use and hybrid facilities, minimizing retrofit costs and supporting future scale-out.
The platform is highly configurable, supporting both cassettes and hollow fiber modules, multiple flow paths, and sensor options. This flexibility enables seamless transitions between different products and batch sizes, reducing downtime and risk of cross-contamination.
Automation reduces material loss and labor dependency, while the closed, sterile design minimizes buffer consumption and cleaning effort. High product recovery and optimized flow profiles further improve profitability for large batch operations.
