Sartoflow® 4500 Commercial-Scale Single-Use Tangential Flow Filtration System

Efficient and Scalable Single-Use System With Automated Processes

The Sartoflow® 4500 single-use tangential flow filtration (SUTFF) system offers user-friendly controls for running fully automated ultrafiltration and diafiltration (UF|DF) processes for batch volumes of 200 to 2000 L (1 to 15 kg).  The system is compact, has low hold-up volume, and the capacity to run cassette surface areas from 3 to 10 m² and hollow fiber modules of 2.69 and 5 m² filtration area (usable filter area is depending on filter type and process parameters).

icon ergonomic hand
Efficient for commercial manufacturing

Designed for efficiency in single-use TFF applications running at production scale

Highly automated

User-friendly process step editor simplifies automation

Flexible and scalable

System offers easy scale-up and flexibility to adapt to changing requirements

Easy to use

Quick single-use flow kit changeout and cleanability minimizes downtime

Sartoflow® 4500 System Concept

The SUTFF system combines hardware, controls and single-use flow kits.

  • A smart user-friendly design simplifies fully automated TFF operations
  • Complete single-use flow kits incorporate critical sensors and instruments
  • Supports automated processing of batch volumes from 200 to 2000 L of monoclonal antibodies, recombinant proteins and vaccines
  • Single-use sensors fully monitor and control the process

Intuitive and Fully Automated Process Control

Automation is made possible by a process step editor, a simple and user-friendly interface for automating operations.

  • Software complies with 21 CFR Part 11
  • Select transition points to advance process steps based on one or more parameters, such as conductivity, retentate volume, permeate volume and bag weight
  • Use loop and jump functions to create selective step repetitions and enhance flexibility
  • Generate batch reports for each step

Ease Scale-Up from Process Development to GMP Commercial Manufacturing

With a unique single-use flow path, the Sartoflow® 4500 features increased turnaround efficiency, reduced cleaning validation, minimized bioburden risk and product carryover in a multi-product facility.

  • Consistent product quality
  • Significantly increased productivity
  • Longer run times, with control and monitoring provided by integrated software

Related Products

TFF Cassettes

Hydrosart® cassettes have a unique membrane with the highest chemical resistance, temperature stability, and gamma compatibility.

Learn More

Learn More About Sartoflow® 4500

General Specification


420 kg

System Dimensions

EMEA | Asia:
1,920 × 1,000 × 1,718 mm (nominal)

1,980 × 1,120 × 1,730 mm (nominal)

System Specification

Membrane Area

up to 10 m² (flat sheet)

2.69 m² and 5 m² (hollow fiber)

Flow Rate / Pump Capacity - Recirculation 

100 – 4,500 L/h

Flow Rate / Pump Capacity - Fed Batch | Buffer Pump

20 - 900 L/h

Volume in Feed and Retentate Lines

2.5 L (with 1" tubing)

Automation Level


Process Mode (Multi-Use / Single-Use)


cGMP available


Max Pressure

4 bar | 58 psi

Wettware Specification

Tube ID

1" feed/retentate




Pressure, flow, conductivity, pH, temperature & UV

Related Assets


Sartoflow® 4500: Single-use Tangential Flow Filtration System

PDF | 859.4 KB
Technical Note

Summary of Extractables from Sartoflow® Single-Use Tangential Flow Filtration (SUTFF) Flow Kits

PDF | 306.7 KB
Application Note

Maximizing Scale and Speed with Intensified Single-Use Tangential Flow Filtration (SUTFF) 

PDF | 1.1 MB

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