Application Note: Pipette Calibration and Standards

Pipetting is an intricate technique, relying on accurate practices and reliable equipment. Pipette performance can deteriorate over time due to drift of calibration, leakage, part wear or contamination. Keeping pipettes and pipette accessories properly maintained is the key to obtaining consistently accurate results in the laboratory.

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Common Questions About Pipette Calibration 

  • Why is pipette calibration important? – Pipette calibration ensures consistent accuracy in dispensing, which minimizes error.
  • How can I check to see if a pipette is properly calibrated? – One of the best and most common ways to check pipette accuracy is to measure a series of pipetting results on an analytical balance. 
  • What is the best way to keep a pipette accurate? – A calibrated pipette will stay accurate longer if it is cleaned and stored properly. A heavily used pipette requires maintenance more often and if dropped any pipette should be checked for damage and performance. 

There are specific protocols for proper calibration and preventive maintenance in pipetting, according to International Standards Organization (ISO) standards, particularly ISO8655 and ISO17025. 

Download this application note to learn more about ISO8655 and ISO17025, as well as:  

  • Good Laboratory Practice (GLP) guidelines
  • Accreditation and Quality Systems  
  • Extending equipment life through maintenance

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