Technical Note:  Incucyte® Live-Cell Imaging and Analysis Best Practices

Cells used in kinetic multi-well assays, within Incucyte® Live-Cell Analysis Systems, typically run for extended time periods. Consequently, cells must be maintained in a healthy state, with sufficient nutrients for the duration of experiments. In addition, appropriate assay set-up parameters, ensure conditions for high quality, reproducible image acquisition of cells over time. 

This technical note highlights the optimization of six parameters for achieving superior assay performance: 

  • Cell Seeding
  • Well Volume Plate Type
  • Assay Media
  • Objects Within Light Path
  • Empty Wells
  • Vessel Positioning


CTA: Download the Technical Note