Application Note: Real-time Live-Cell Analysis of 3D Organoid Growth in Matrigel® Domes
Overview
Recent advances in organoid technology have opened up new horizons for translational human disease research, disease modeling, regenerative medicine and predictive precision therapies.
Organoids are differentiated primary micro tissues formed from a variety of stem cells (SCs) that can be established within 3D extracellular matrices to mimic in-vivo architecture and genetic diversity. As self-organizing and self-renewing structures, organoids have a distinct advantage over traditional monolayer culture techniques and hold unprecedented potential for various applications.
In order to effectively use these models in basic research, disease modeling and drug screening specific and reliable in-vitro culture and analysis methods are required.
Currently, characterization and optimization of organoid cultures are limited in their ability to objectively monitor these 3D structures as they form and grow over time. The Incucyte® Organoid Analysis Software Module provides a solution to standardize and automate organoid culture workflows, simplifying culture characterization and optimization.
- Document type: Application Note
- Page count: 4
- Read time: 5 minutes
Key Takeaways
Download this Application Note to review:
- live-cell analysis to study the formation and growth of organoid cultures
- monitoring and quantifying organoid growth in Matrigel® domes
- optimization and characterization of organoid cultures using real-time kinetic measurements
