Application Note: Long-Term Live Cell Visualization and Quantification of Neuronal Activity
A major impediment to studying diseases affecting the human nervous system is the ability to monitor, analyze, and quantify the activity of neuronal cell populations that accurately represent human phenotypes.
With the advent of cellular reprogramming technologies, there has been abundant research toward protocol development to differentiate human induced pluripotent stem cells (hiPSCs) into multiple cell populations found in the brain (e.g. neuronal, glial, immunological, etc.). This has resulted in the generation of many different neuronal cell models (e.g. dopaminergic, GABAergic, glutamatergic, peripheral, etc.), most of which remain poorly characterized. This imparts a requirement to better understand in vitro cellular models and identify means by which they could be refined.
The IncuCyte® S3 Live-Cell Analysis System for Neuroscience technology, methodology, and applications described within this application note were designed with these issues in mind. That is, to provide researchers with a set of automated tools in order to facilitate the evaluation, characterization, and validation of complex neuronal models.