Application Note: Filtration of Culture Supernatants of Pathogenic Bacteria Using Sartoclear Dynamics® Lab
Authors: Gina Porras, Shubham Joge, Thomas Böttcher | Last updated: Feb. 2024
Overview
The application note explores the use of the Sartoclear Dynamics® Lab filtration kits for the clarification of high-density human-associated bacteria supernatants, specifically Pseudomonas aeruginosa. The study evaluates the system's ability to improve filtration speed and examines its effect on the chemical profile of crude extracts. The results indicate that the Sartoclear Dynamics® Lab can filter bacteria supernatants efficiently without prior centrifugation and does not alter the profile of secondary metabolites secreted by the microorganisms.
- Document type: Application Note
- Page count: 4
- Read time: 10 minutes
Key Takeaways
- Improved Filtration Efficiency: The Sartoclear Dynamics® Lab system significantly improves the filtration process of moderate volumes of human bacteria supernatants by decreasing both the filtration time and the number of filters required, without the need for a prior centrifugation step.
- Chemical Profile Integrity: The use of the Sartoclear Dynamics® Lab system does not generate alterations in the chemical profile of the samples analyzed, ensuring that the subsequent molecule isolation process will not be affected.
- Potential Elimination of Centrifugation: The addition of diatomaceous earth (DE) into both centrifuged and non-centrifuged bacterial supernatants significantly decreased filtration times and inhibited rapid filter clogging, suggesting that the use of filter aid could eliminate the need for centrifugation in the workflow, thereby shortening the processing time for each sample.
- Yield Considerations: While the isolated amount of the target metabolite was slightly lower than expected, these differences cannot be directly attributed to the use of filter aid. Variations in yields frequently occur when working with extracts from natural sources such as microorganisms, where small changes in growth conditions can affect the production of a specific molecule.
This Resource is Designed for:
Lab Researchers
Microbiologists and Biotechnologists
Pharmaceutical researchers
Laboratory Technicians
Academic Researchers
Clinical Researcher
Process Engineers
Applications Supported:
- Preparation of cell-free supernatants
- Filtration of high-density bacteria supernatants
- Improving filtration workflow
- Elimination of centrifugation step
