Optimized Media Solutions for Your hiPSC Suspension Culture
Nutri3D hiPSC ACF is an animal component-free medium for efficient and scalable hiPSC expansion from 2D seed train to 3D aggregate suspension culture, supporting high-density growth and consistent cell quality.
Single-medium for 2D seed train to 3D scale-up
Animal component-free (ACF) and defined formulation
Manufactured under applicable cGMP guidelines
DMF available soon
Nutri3D hiPSC ACF Medium is available in bottle and single-use bag formats to support closed-system cell culture in bioreactors using aseptically weldable TPE tubing. The medium and final packaged products are manufactured in accordance with cGMP principles. With no media switch required, Nutri3D hiPSC ACF supports flexible, seamless scale-up from 2D seed train to 3D large-scale manufacturing - lowering qualification hurdles and simplifying the transition from R&D to production.
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Nutri3D hiPSC ACF Medium Delivers Leading 2D hiPSCs Expansion
Nutri3D hiPSC ACF enables robust 2D expansion of human iPSCs under defined, animal component-free conditions. Across multiple passages and matrix conditions, independent hiPSC lines cultured on Laminin-521 (LN-521) or Vitronectin (VTN) showed improved expansion performance compared with alternative 2D ACF and xeno-free commercially media.
Nutri3D hiPSC ACF Shows Enhanced 3D Expansion in Shake Flasks
Nutri3D hiPSC ACF Medium achieved higher 3D aggregate expansion in shake flasks compared with two commercial 3D iPSC media across three independent hiPSC lines. Culture conditions followed each manufacturer’s instructions, including optional surfactant addition.
hiPSCs Expanded on Nutri3D hiPSC ACF Exhibit Uniform Aggregate Morphology
Nutri3D hiPSC ACF supports robust 3D expansion of human iPSCs under defined, animal component-free conditions, maintaining consistent aggregate morphology, circularity, and size across passages.
Nutri3D hiPSC ACF Outperforms Commercial Alternative ACF Product in 3D Expansion
Nutri3D hiPSC ACF enables efficient 3D aggregate expansion of human iPSCs in bioreactors. Performance was demonstrated in a vertical-wheel system, with hiPSC aggregate expansion benchmarked against a commercially available alternative under conditions without shear protectant (PF-68) supplementation.
Cells Expanded in Nutri3D hiPSC ACF Retain Full Differentiation Potential
iPSCs expanded in Nutri3D hiPSC ACF using different 2D and 3D platforms were differentiated into ectodermal, mesodermal, and endodermal lineages. Flow cytometry confirmed high expression of lineage-specific markers.
