Application Note: Biomolecular Binding Kinetics Assays on the Octet® Platform
Overview
Detecting and quantifying biomolecular interactions are critical in the discovery and selection of biotherapeutics leads. Octet® Bio-Layer Interferometry (BLI) systems implement a label-free analytical approach, which provides a fast and straightforward means to accurately determine binding specificity, affinity, and kinetics, which can be useful in every stage of biopharmaceutical development from early discovery to manufacturing.
This application note introduces the family of Octet® BLI systems, the technology, capabilities, and advantages of label-free binding assessment in therapeutics development and life sciences research. It also provides guidance and best practices in biosensor assay development, optimization, data analysis, and reporting.
- Document type: Application Note
- Page count: 28
- Read time: 75 minutes
Key Takeaways:
- Principles and essential elements involved in performing successful large molecule kinetic assays on the Octet® BLI platform
- Importance of biosensor selection
- Proper assay development to ensure 1:1 binding
- Appropriate range of analyte concentrations and steps to minimize non-specific binding heterogeneity
- Key considerations regarding method of immobilization, assay orientation, and choice of buffer
- Optimization of ligand loading to avoid binding artifacts
- Yielding accurate, reproducible data and reliable kinetic and affinity constants
Workflow Supported
Kinetic assays are used throughout biopharmaceutical development including:
- Discovery
- Process Development
- Manufacturing
- Quality Control
This resource is designed for researchers working in the areas of:
- Target ID and Validation
- Lead Screening and Selection
- Lead Optimization and Characterization
- Upstream Process Development
- Downstream Process Development
- Manufacturing
- Quality Control
