Biomolecular Binding Kinetics Assays on the Octet® Platform
Detecting and quantifying biomolecular interactions are critical in the discovery and selection of biotherapeutics leads. Octet® systems implement a label-free analytical approach utilizing Bio-layer interferometry (BLI), which provides a fast and straightforward means to accurately determine binding specificity, affinity, and kinetics, which can be useful in every stage of biopharmaceutical development from early discovery to manufacturing.
This application note introduces the Octet® family of label-free platforms, the technology, capabilities, and advantages of label-free binding assessment in therapeutics development and life sciences research. It also provides guidance and best practices in biosensor assay development, optimization, data analysis, and reporting.