Effectively Use Picus® Pipettes with Vivaspin® Concentrators - Application Note

Use of centrifugal concentrators for buffer exchange or sample concentration requires optimization of the protocol and correct handling of the device in conjunction with a pipette to ensure:

  • Determination of the concentrated sample volume for accurate yield measurement
  • Retrieve the full volume of sample in a tall concentrator tube while avoiding sample-to-pipette and pipette-to-sample contamination
  • Proper rinsing so that the preservatives used to maintain membrane stability do not elute into the sample leading to anomalous peaks in concentrate analysis Sewage surveillance of COVID-19 prevalence in communities requires concentration of viral material from large sample volumes.

In this practical guide, we provide guidance on the efficient use of electronic pipettes and extended length pipette tips with when concentrating RNA from COVID-19 wastewater surveillance samples.

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