Top 10 Tips to Avoid Contamination in Pipetting 

Do you know how to avoid contamination in pipetting? One of the most essential – but overlooked – methods of cell culture contamination prevention is proper aseptic pipetting technique. The risk of contamination can be kept to a minimum by always employing the best maintenance practices. 

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Commonly Asked Questions:  

  • How can pipette contamination be prevented?  - Contamination of the pipette itself can be avoided by using sterile tips of proper length and employing slow dispensing methods to avoid splashes. When not in use, contamination can be avoided with regular cleaning and proper storage stands.
  • Which pipette handling errors are common? 

    • Choosing the wrong mode between forward and reverse pipetting
    • Unnecessary wiping of pipette tips 
    • Failure to pre-wet pipette tip 
    • Not factoring sample temperature 
    • Improper pipette cleaning and maintenance  
    • Not accounting for liquid viscosity 
  • What is the best way to decontaminate a pipette?  - Proper pipette decontamination depends on a variety of factors. When choosing cleaning and decontamination solutions for your equipment, consider the liquid or reagent that has been pipetted. You should also ensure the solutions will be compatible with the pipette materials. An in-depth tutorial for proper pipette cleaning and decontamination can be accessed here.  
  • Which types of contamination can occur with improper pipetting?

    • Pipette-to-sample: Pipettes or pipette tips transfer contaminants to a sample 
    • Sample-to-pipette: Contaminated samples enter the pipette body  
    • Sample-to-sample: Residual materials from a pipetted sample are carried over to a different sample 

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