Solutions for Culturing, Maintaining and Characterizing iPSCs
Authors: Daryl Cole, PhD, Kirsty McBain, Nicola Bevan | Last updated: August 2023
Overview
From drug discovery to organoid modeling of disease, stem cells are increasingly being used in research as a vital tool for scientific investigation. The current trend away from animal models and the push to more relevant systems for simulating the human body require flexible and specific tools to achieve this goal. Induced Pluripotent Stem Cells (iPSCs) are produced from normal tissue, through the forced expression of key transcription factors, providing a limitless supply of these precious cells for research and development. Due to the very specialized nature of these cells, their maintenance and culture is more intensive than most cell lines. Characterization of stem cells can be difficult and unreliable, depending on the methodology used, which is why it is important to develop robust techniques for monitoring stem cells throughout culture and experimental testing. If conditions are not optimal during the maintenance of iPSCs, their pluripotency can be lost.
This application note showcases the advantages of using a streamlined workflow combining multiple Sartorius systems for the culture, monitoring and characterization of iPSCs for several applications from drug development, disease modeling and clinical therapy research.
- Document type: Application Note
- Page count: 8
- Read time: 15 minutes
Key Takeaways
Consistently and reliably pick specific iPSC colonies for further testing or culture while maintaining high levels of cell health
Monitor delicate iPSC line culture morphology and growth characteristics without removing plates from the incubator
Use minimal sample volumes required to characterize precious cell types, with minimal attrition for downstream requirements
Multiplexing experiments providing flexibility for the characterization of multiple metrics, such as surface and intracellular marker expression, using the same platform
Solutions for the Culturing, Maintaining and Characterization of Induced Pluripotent Stem Cells
