Hydrophobic Interaction Chromatography (HIC)
Hydrophobic interaction chromatography (HIC) is used to separate molecules based on the differences of their surface hydrophobicity. This technology is widely used in chromatographic purification. It is orthogonal to other techniques like ion exchange or affinity. Hydrophobic interaction chromatography commonly requires high salt concentration for efficient binding of the target protein. Therefore, it is ideally used after chromatographic steps where elution is accomplished with high salt concentrations, i.e. ion exchange chromatography.
The HIC Membrane Can Be Used for the Removal Of:
- Aggregates
- Host cell proteins
- Viruses
- Endotoxins
- Lipids, dyes, detergents and anti foam agents
- Leached chromatography ligands
Easy to Use:
- Plug and play devices from 0.08 ml to 5 l
- Can be used like a filter
- Flow rate up to 5 bed volumes/min
- Re-usable or disposable
