Hydrophobic Interaction Chromatography (HIC)

Hydrophobic interaction chromatography (HIC) is used to separate molecules based on the differences of their surface hydrophobicity. This technology is widely used in chromatographic purification. It is orthogonal to other techniques like ion exchange or affinity. Hydrophobic interaction chromatography commonly requires high salt concentration for efficient binding of the target protein. Therefore, it is ideally used after chromatographic steps where elution is accomplished with high salt concentrations, i.e. ion exchange chromatography.

The HIC Membrane Can Be Used for the Removal Of:​

  • Aggregates​
  • Host cell proteins​
  • Viruses​
  • Endotoxins​
  • Lipids, dyes, detergents and anti foam agents​
  • Leached chromatography ligands​

Easy to Use:​

  • Plug and play devices from 0.08 ml to 5 l​
  • Can be used like a filter​
  • Flow rate up to 5 bed volumes/min​
  • Re-usable or disposable​
Process Chromatography

Monoliths for Preparative and Analytical Chromatography

The right chromatography tools for the right tasks - and the skills to use them.

Learn More

Request a Quote  

Related Content


Virus Clearance Validation

Mitigate the risks of low LRV results in GLP studies with Confidence® Virus Clearance Services, which eliminate the top reasons for study failure.

Learn More

Process Filtration

Learn More