Live-Cell Monitoring: Optimizing Workflows for Advanced Cell Models
Authors: Thomas Dennison, Research Associate, Milner Therapeutics Institute & Zilbauer Lab, Cambridge Stem Cell Institute | Last updated: November, 2025
Overview
In this article with Thomas Dennison from the Cambridge Stem Cell Institute, we explore the innovative use of live-cell imaging in CRISPR screening with intestinal organoids, focusing on Inflammatory Bowel Disease (IBD) research. By utilizing patient-derived organoids, researchers can better understand the disease's complexity and diversity, identifying unique molecular signatures. The Incucyte® Live-Cell Analysis System facilitates continuous monitoring and high-throughput data collection, preserving the 3D structure of organoids. This approach enhances data accuracy and objectivity in image analysis. The combination of CRISPR screening and live-cell imaging offers potential for discovering new therapeutic targets and biomarkers, with future advancements in technology promising further insights into complex diseases like IBD.
- Document type: Article
- Page count: 4
- Read time: 10 minutes
Key Takeaways
- Innovative Research Approach: Utilizes live-cell imaging in CRISPR screening with intestinal organoids to study Inflammatory Bowel Disease (IBD).
- Enhanced Data Collection: The Incucyte® Live-Cell Analysis System allows continuous monitoring and high-throughput data collection while preserving organoid structures.
- Potential for Therapeutic Discovery: Combines CRISPR screening and live-cell imaging to identify novel therapeutic targets and prognostic biomarkers.
- Future Technological Advancements: Anticipates improvements like real-time fluorescence readouts and AI-driven image analysis to deepen insights into complex diseases.
