Application Note: Development and Optimization of Corning® Matrigel® 3D Assays
Last updated: January 2024
Overview
Research into spheroids and organoids is forging new paths in human disease research, disease modeling, regenerative medicine, and the development of targeted, precise treatments. These 3D microtissues and organoids offer insights that more closely mimic human physiology than traditional 2D cell cultures, which are often cultivated on artificial surfaces and in conditions that can skew the results of drug efficacy or metabolic studies.
Despite their potential, the analysis and refinement of organoid cultures have been hindered by the lack of methods to monitor the development and growth of these complex 3D structures objectively and continuously.
Incucyte® Organoid and Incucyte® Single Spheroid assays are designed to overcome these obstacles in 3D organoid and spheroid research. By combining an optimized assay protocol with Corning microplates and Incucyte® Live-Cell Analysis software modules, these assays facilitate real-time, reliable, and multiparametric monitoring of organoids and spheroids under conditions that closely resemble human physiology.
- Document type: Application Note
- Page count: 7
- Read time: 8 minutes
Key Takeaways
- Single Spheroid Assays and Supporting ULA Surface, Round Bottom Microplates
- Multi-Spheroid (MS) Assay Reproducing the Tumor Microenvironment in 3D Culture Models
- Organoid Culture QC and Organoid Assays
Figure 1. MDA-MB-231 spheroid toxicity after exposure to CPT. (A) Representative images of MDA-MB-231 spheroids exposed to vehicle control (top) or CPT
treatment (bottom). (B) Dose response curves from MDA-MB-231 spheroid area after exposure to CPT or vehicle control.
