How to Purify mAbs Using Sartobind® Rapid A Lab (Fast) — Application Guide
Authors: Benjamin Graf, Lina Miethke, John Cashman | Last updated: August 2024
Overview
Immunoglobulins are the cornerstone of the adaptive immune system and a key molecule for modern medicine. The specificity, versatility, longevity, and proven track record of monoclonal antibodies (mAbs) make these Fc-containing molecules in particular ideal for a wide range of therapeutic and diagnostic applications. Following production in mammalian, bacterial or plant cell cultures, mAbs are purified and processed in a relatively standardized workflow. However, legacy separation technologies are labor intensive, slow and result in limited yields.
In this guide, we present a cutting-edge mAb purification process that encompasses the entire workflow from cell culture clarification to terminal sterile filtration. In addition to our other innovative separation solutions, you will learn how our new protein A affinity membrane chromatography unit, Sartobind® Rapid A Lab, perfectly fits into this workflow to quickly purify mAb samples with a high yield.
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- Document type: Application Guide
- Page count: 8
- Read time: 9 minutes
Key Takeaways
- See how Sartorius separation products can be used together in a cutting-edge mAb purification workflow
- Learn how our new protein A affinity chromatography unit, Sartobind® Rapid A Lab, enables effective and reliable purification of mAbs
- Understand the advantages of membrane affinity chromatography for antibody capture
- Recognize how our innovative separation solutions can significantly speed up the mAb purification process.
This Resource is Designed for:
- Research Scientist
- Process Development Scientist
- Antibody Engineering Scientist
Applications Supported:
- Antibody purification workflow
- Affinity membrane chromatography
- mAbs
Application Guide Preview
Figure 1. Cover image of the mAb Purification Process Application Guide.
