Equipment-Free Macromolecule Purification: Using Sartobind® Lab Units with a Syringe
Last updated: February 2023
Overview
This protocol describes how to operate Sartobind® Lab units manually, with a syringe. Unlike resin-based columns, the macroporous structure of Sartobind® membrane adsorbers enables rapid transport of macromolecules to the functionalized surface by convective flow. This supports reliable, equipment-free purifications by syringe at much faster flow rates and shorter residence times, without exceeding pressure limits or compromising target molecule yields.
- Document type: Protocol
- Page count: 3
- Read time: 3 minutes
Key Takeaways
In this protocol, you will find:
- A list of required materials
- General guidelines for handling Sartobind® Lab units with a syringe
- Visual overview of the capture-wash-elute process
- Step-by-step written instructions
- Hints to speed up your process
This Resource is Designed for:
- Lab researchers
- PhD students
- Research technicians
- Research associates
- Process development scientists
Applications Supported:
- Purification
- Ion exchange chromatography
- Affinity chromatography
- Capture purification
- Protein purification
- Virus purification
- Nucleic acid purification
- Crystallography
- Structure-function studies
Figure 1. Visual step-by-step instructions for syringe-driven purifications
