Monitor and Characterize iPSC Culture and Differentiation, using Advanced Flow Cytometry
Authors: Dr. Daryl Cole, Scientist, Analytics, Sartorius | Last updated: 13 September 2023
iQue® Poster Presentation Snapshots: Monitor and Characterize iPSC culture and Differentiation using Advanced Flow Cytometry and Live-Cell Analysis
Induced pluripotent stem cells (iPSCs) are intrinsically valuable due to their unique characteristics and the control they afford to enable researchers to investigate early stages in cellular development.
The major benefits of iPSCs are the variety of cell types they can be differentiated into and their capacity for infinite expansion. This flexibility provides opportunities for development of cell and tissue models in both 2D and 3D for pharmacological testing, cancer, research, organoid modelling of tissues and neurodevelopmental biology.
iPSCs are increasingly used in translational applications, targeting eventual clinical use via autologous cell therapies and individualized medicine approaches.
Despite their broad application potential, iPSCs are high maintenance, expensive, and require constant monitoring to ensure they maintain pluripotency, viability, and homogeneity.
Poster Podcast Key Takeaways:
- Demonstration of how to evaluate and monitor the pluripotency of iPSCs both in 2D and 3D culture systems, by cell surface marker evaluation using the iQue® Advanced Flow Cytometry Platform and morphological analysis using an Incucyte® Live-Cell Analysis System.
- Description of how long-term hepatic iPSC differentiation experiments can also be successfully monitored and validated throughout the time-course, providing a streamlined solution for differentiation quality control experiments.
- Explanation of how the maintenance of pluripotent phenotype can be achieved without daily media changes, using Sartorius® Research Use Only (RUO) growth factors and cytokines.
