Functional Analysis of Antibodies: An Advanced Flow Cytometry Poster Compendium
Overview
Innovation in hybridoma and phage display technologies, have allowed drug developers to exploit the natural diversity in antibodies, leading to the wide development of monoclonal antibodies (mAbs) and other antibody-based proteins for treating cancers, chronic inflammation, and infectious diseases.
Antibody discovery programs rely on high throughput technologies to quickly identify candidates with therapeutic potential. The process begins with identifying thousands of antibody candidates against a specific target, and then triaging them through a series of in vitro binding and functional assays to identify the top leads.
In this compendium, we handpicked nine posters that demonstrate how the iQue® Advanced Flow Cytometry Platform can accelerate antibody discovery and development via rapid, multiplexed analysis of parameters within a single sample well. A key differentiator of the iQue® platform is powerful integrated software, which dramatically simplifies interpretation of complex datasets.
Studies include routine characterizations for binding, antibody internalization, antibody-dependent cellular phagocytosis (ADCP), antibody-dependent cell-mediated cytotoxicity (ADCC), and T-cell activation involving mAbs and antibody-derived biologics Bi-specific T-cell Engager (BiTE) antibodies and antibody-drug conjugates (ADCs).
- Document type: Poster Compendium
- Page count: 12
- Read time: 30 minutes
Key Takeaways
Topics include:
- Binding and functional characterization of mAbs
- Accelerating ADCP screening
- Characterization of BiTE antibodies
- Multiplexed antibody internalization assay
- Assessment of NK cell-mediated killing
