Article Collection: Advanced Flow Cytometry for Stem Cells
Overview
Induced pluripotent stem cells (iPSCs), derived from numerous sources such as primary fibroblasts or hematopoietic stem cells (HSCs), are rapidly becoming the gold standard model system for drug development, as well as a scalable source for cell-based therapies.
Characterization of iPSCs to ensure quality and cell fate are key to their use. Advanced flow cytometry methods are an instrumental part of the characterization process, able to accurately and efficiently phenotype cells during different stages of derivation and expansion.
In this article collection, we highlight publications that illustrate the use of pluripotent stem cells for cell-based therapies and how flow cytometry can be utilized to measure cell parameters.
Author groups include Vladimira and Bojanić, Du et al., Kim et al. and Torizal et al.
Page count: 60
Read time: 100 Minutes
Key Takeaways
The eBook includes the following chapters from experts in the field:
- Role of flow cytometry in evaluation of the cellular therapy products used in haematopoietic stem cell transplantation
- Novel strategy to improve hepatocyte differentiation stability through synchronized behavior-driven mechanical memory of iPSCs
- Generation of universal natural killer cells from a cryopreserved cord blood mononuclear cell-derived induced pluripotent stem cell library
- Production of homogenous size-controlled human induced pluripotent stem cell aggregates using ring-shaped culture vessel
- Characterization and Optimization of Induced Pluripotent Stem Cell Culture Using Advanced Flow Cytometry and Live-Cell Analysis
