On-Demand Webinar: The Need for Speed: Nanowell-Based Cloning for Viral Vector Production
Mammalian cell platforms offer many advantages for viral vector and recombinant protein production, with cell lines such as HEK293 able to be expanded for the large-scale production of bioproducts.
As part of this process, researchers must generate stable producer cell lines, which can be challenging using standard methods such as limiting dilution and cell sorting.
Watch as Sophie Broussau of the National Research Council (NRC) in Canada demonstrates how to accelerate the development of high-producing HEK 293SF-3F6 lentivirus packaging cell lines.
- Watch time: 61 minutes
- Learn how to develop a stable lentivirus packaging cell line
- Hear about the experimental data that demonstrates the monoclonality of cell lines produced by a new method
- Discover the CellCelector Flex Nanowell cloning system and how it can benefit your research
Cell Line Development Specialist
National Research Council, Canada
Sophie Broussau, M.Sc., is a technical officer at the National Research Council (NRC) in Canada. Over the past 20 years, she has been working on the development of several HEK 293SF-3F6 derived stable cell lines for lentiviral vector and recombinant protein production for R&D and clinical applications. Currently, she is developing a cloning method to improve the isolation of high-performing clones. She has extensive experience in small- and large-scale cell culture, lentiviral production, molecular biology, cytometry, and recombinant protein production and purification.