Overview
In gene therapy, recombinant adeno-associated viruses (rAAV) serve as ideal vectors for gene delivery, due to their minimal pathogenicity and long-term gene expression capabilities.
This application note highlights a high-throughput method for single-cell cloning, enhancing the efficiency of rAAV production. Automated screening of 400 clones using the CellCelector Flex Platform, which significantly reduces resource requirements and accelerates cell line development, is detailed. Subsequently reducing resource requirements and accelerating cell line development.
Key findings indicate that while genomic titer is influenced by cell clone, transfection reagent, and plasmid set, the proportion of full viral particles is affected by the plasmid set, transfection reagent, and feeding strategy.
Resource Details
- Document Type: Application Note
- Page Count: 12
- Read Time: 15 minutes
Key Takeaways
The study explores the optimization of rAAV yield through:
- Various plasmid sets
- Serotypes
- Transfection agents
- Feeds
- Culture media