Resource Overview
In the field of regenerative medicine, the formation of multi-lineage heart-forming organoids (HFOs) from human pluripotent stem cell (hPSC) aggregates, represents a significant development. Although multilineage organoids provide an expanded window into development and disease, there are increases in the complexity of their production.
Manual identification, selection and isolation of high-quality hPSC aggregates, for differentiation into HFOs, has drawbacks. In addition, when using manual techniques, Matrigel® embedding, and the lack of speed of data generation, can cause batch-to-batch inconsistencies.
This case study presents a novel workflow that automates the analysis and embedding of hPSC aggregates in Matrigel®, enabling simultaneous quality control prior to embedding, and ensuring high quality organoid patterns while maintaining viability comparable to manual processes.
The utility of the CellCelector Flex, in the automation of identification, selection and isolation of high-quality hPSC aggregates, for differentiation into HFOs, is also highlighted.
Resource Details:
- Document Type: Case Study
- Page Count: 6
- Read Time: 10 minutes
Key Takeaways
Includes details of:
- Optimizing picking settings for successful embedding of optimal aggregates
- Selecting optimal aggregate properties for HFO production