Second Generation Anti-Murine IgG Capture Biosensors (AMC2) Designed for Kinetic Characterization of Murine IgG – Antigen Interactions Application Note
Authors: Dan Su, PhD, Stuart Knowling, PhD, Ivan Krylov, PhD, Ling Zhang, PhD, David Apiyo, PhD, Bob Dass, PhD | Last updated: May 2023
Overview
The second-generation Octet® AMC2 Biosensors enable scientists to extend the usage of the anti-mouse capture biosensors to applications such as measurement of high antibody titer (a critical quality attribute), hybridoma screening, kinetic characterization, epitope binning or diversity assays. This application note discusses how the Octet® AMC2 Biosensor can support different stages in antibody development workflows such as early phase discovery, cell-line development, process development and manufacturing. AMC2 Biosensors were developed to serve as a single biosensor for use in both quantitation and kinetics applications. Further, the application note presents and discusses the results of a series of experiments performed at Sartorius and customer sites evaluating the performance of the AMC2 Biosensors and comparing their use with the first generation anti-mouse biosensor (AMC Biosensors) in kinetics applications.
- Document type: Application Note
- Page count: 17
- Read time: 30 minutes
Key Takeaways
Key features of Octet® AMC2 Biosensors include:
- Rapid quantitation and kinetic analysis of both crude and purified samples
- High specificity to all mouse and rat IgG subclasses
- Increased biosensor ligand loading capacity for improved sensitivity
- Wide dynamic range: 0.025 – 8,000 µg/mL
- Efficient and cost-effective biosensor regeneration
- Precision of quantitation: CV <10%
