Ultrapure Water for HPLC Analysis - Application Note

The Role of Ultrapure Water

HPLC is an analytical procedure for separation, identification and quantification of substances using liquid chromatography. The beginnings of HPLC – High Pressure Liquid Chromatography – go back to the 60’s. Thanks to improved column materials and equipment, it has come to be known as High Performance Liquid Chromatography since the end of the 70’s.

In HPLC, the mixture to be separated is transferred to a column with a solvent (eluting agent) or with a solvent mixture (eluant|mobile phase), by an injector and a pump. The column is a tube, in most cases of stainless steel, filled with the so-called stationary phase. The stationary phase usually consists of porous silica gel or polymer particles with chemical ligands bound on their surface. These ligands are responsible for the selective interactions between the analytes and the stationary phase, which are necessary for effective chromatographic separation. Depending on the sample and stationary phase, the separation mechanisms involved are, for example, adsorption by Van der Waals forces, ion exchange, ion exclusion, etc.

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